GE-ASSOSIATED ASPECTS OF THE EXPRESSION OF HUMAN ENDOTHELIAL SIGNAING MOLECULES AND THEIR ROLE IN THE DEVELOPMENT OF CARDIO-VASCULAR PATHOLOGY

DOI: https://doi.org/None

V.M. Soldatov (1), K.L. Kozlov (1), N.S. Linkova (1,2) 1 -Saint Petersburg Institute of Bioregulation and Gerontology, Dunamo pr, 3, Saint Petersburg, 197110, Russian Federation; 2 -Peter the Great Saint Petersburg Polytechnic University, Polytechnicheskaya str., 29, Saint Petersburg, 195251, Russian Federation

Introduction. Age-assosiated endothelial changes are one of the reasons of the development of cardio-vascular pathology. Moreover, molecular mechanisms of human endothelium aging are investigated only slightly. Thus, the problem of pathogenesis of cardio-vascular diseases is not clear yet. The goal of this work is investigation of age-dependent peculiarities of the expression of human endothelial signaling molecules. Methods. There was used autopsy material of the aorta of young, middle, elderly, senile and long-liver patients. For investigation of endothelial signal molecules expression there was used immunohistochemistry method with morphometry for the quantitative data analysis. Results. VCAM-1 and Р-selectin expression has increased by 4 times in endothelial cells of elderly and old people. The expression of PCNA proliferative protein in endothelium of elderly, old and long-liver patients decreased by 3,7 times and expression of proapoptotic protein Caspase-3 increased by 3,5 times. The expression Histone deacetilases Sirt1 and Sirt6 was decreased by 5 and 3 times in endothelium of elderly, old and longliver patients in comparison with endothelium of young and middle-aged cases. Conclusion. People older 60 years were shown to have the decreasing of functional activity and cell renovation of vessel endothelium. Moreover, endothelium adhesion ability is decreasing during aging. Age-dependent decreasing of sirtuins expression is the marker of endothelium cell senescence, which was characterized by accumulation of DNA damage. It is shown the increasing of cardio-vascular pathology risk probability in elderly and old people. Signaling molecules, which were described in this article, can be used as molecular markers for predictive diagnostics of cardio-vascular pathology.
Keywords: 
vessels, immunohistochemistry, aging, signaling molecules
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