THE SPONTANEOUS DELIVERY AFTER CORRECTION OF HYDROMETRA BY ADMINISTRATION OF MULTIPOTENT MESENCHYMAL STROMAL CELLS IN EXPERIMENT

DOI: https://doi.org/None

I.V. Maiborodin (1), N.V. Onoprienko (2), O.G. Pekarev (2), G.A. Chastikin (1), V.A. Matveeva (1) 1 -The Center of New Medical Technologies, Institute of Chemical Biology and Fundamental Medicine, ak. Lavrent’eva str., 8, Novosibirsk, 630090, Russian Federation; 2 -Novosibirsk State Medical University, Russia, Krasny Prospect, 52, Novosibirsk, 630091, Russian Federation

Introduction. The angiogenesis in a uterus scar after application of multipotent mesenchymal stromal cells of a bone marrow origin (MMSCBM) was shown. However, possibility of approach of pregnancy and spontaneous deliveries in rats after MMSCBM introduction in places of an atrophy of all layers of a uterus was not studied. The aim of the study. To study changes in tissues of rat uterus and investigate possibility of spontaneous delivery after modeling of hydrometra with the subsequent introduction of MMSCBM. Material and methods. The possibility of approach of pregnancy and delivery and also changes in uterus tissues after MMSCBM with transfected GFP gene use after modeling hydrometra in rat were studied. Results and discussion. After an injection of MMSCBM in a uterus scar the number of the vessels formed de novo from entered cells increases. Vascular walls or their separate elements were constructed with MMSCBM. After MMSCBM application the animals started to give labor by 2 oestral cycle earlier, the percent of the given rise in rats from this group was higher, they had more both a posterity total, and the maximum number of infant rats. Maternal mortality after MMSCBM application, on the contrary, was less. Conclusion. After introduction of MMSCBM there is a sure tendency to acceleration of the reparative processes in a rat uterus in cicatricial narrowing of its cavity.
Keywords: 
uterus scar, hydrometra, multipotent mesenchymal stromal cells, angiogenesis
Список литературы: 
  1. Mayborodin I.V., Yakimova N.V., Matveeva V.A., Pekarev O.G., Mayborodina E.I., Pekareva E.O., Tkachuk O.K. Angiogenez kak rezul`tat vvedeniya mezenhimal`nyh stvolovyh kletok v rubec matki krys. Molekulyarnaya medicina. 2011; (4): 28–35. [Maiborodin I.V. Yakimova N. V., Matveev V.A. Pekarev O. G., Mayborodina E.I., Pekareva E.O., Tkachuk O.K. Angiogenesis as result of introduction of mesenchymal stem cells in a scar of a rat uterus. Molekuljarnaja medicina. 2011; (4): 28–35 (in Russian)]
  2. Saharov P.P., Metelkin A.I., Gudkova E.I. Laboratornye zhivotnye. M.: Medgiz, 1952; 316. [Sakharov P.P., Metelkin A.I. Gudkova E.I. Laboratory animals. Moscow: Medgiz, 1952; 316 (in Russian)]
  3. Leyn-Petter U. Obespechenie nauchnyh issledovaniy laboratornymi zhivotnymi. M.: Medicina, 1964; 194. [Lane-Petter U. Ensuring scientific researches with laboratory animals. Moscow: Medicine, 1964; 194 (in Russian)]
  4. Zapadnyuk I.P., Zapadnyuk V.I., Zahariya E.A., Zapadnyuk B.V. Laboratornye zhivotnye. Kiev: Vishha shkola; 1983; 383. [Zapadnyuk I.P., Zapadnyuk V. I., Zachariah E.A. Zapadnyuk B. V. Laboratory animals. Kiev: Higher school; 1983; 383 (in Russian)]
  5. Wu X., Pan L., Wang Z., Liu X., Zhao D., Zhang X., Rupp R.A., Xu J. Ultraviolet irradiation induces autofluorescence enhancement via production of reactive oxygen species and photodecomposition in erythrocytes. Biochem. Biophys. Res. Commun. 2010; 396 (4): 999–1005.
  6. Campo J.J., Aponte J.J., Nhabomba A.J., Sacarlal J., Angulo-Barturen I., Jiménez-Díaz M.B., Alonso P.L., Dobaño C. Feasibility of flow cytometry for measurements of Plasmodium falciparum parasite burden in studies in areas of malaria endemicity by use of bidimensional assessment of YOYO-1 and autofluorescence. J. Clin. Microbiol. 2011; 49 (3): 968–74.
  7. Watson J. Suppressing autofluorescence of erythrocytes. Biotech. Histochem. 2011; 86 (3): 207.
  8. Tsuji T, Sawabe M. Elastic fibers in striae distensae. J Cutan Pathol. 1988; 15 (4): 215–22.
  9. Mayborodin I.V., Shevela A.I., Babko A.N., Morozov V.V., Zagoruyko T.Yu., Matveeva V.A. Morfologicheskie rezul`taty primeneniya kletochnyh tehnologiy dlya korrekcii lineynoy atrofii kozhi. Rossiyskiy zhurnal kozhnyh i venericheskih bolezney. 2010; (1): 57–62. [Maiborodin I.V. Shevela A.I. Babko A.N., Morozov V. V., Zagoruyko T.Yu., Matveeva V.A. Morphological results of application of cellular technologies for correction of the linear atrophy of skin. Rossijskij zhurnal kozhnyh i venericheskih boleznej. 2010; (1): 57–62 (in Russian)]
  10. Satli M.A., Aron C. New data on olfactory control of estral receptivity of female rats. C. R. Acad. Sci. Hebd. Seances Acad. Sci. D. 1976; 282 (9): 875–7.
  11. Babichev V.N., Ignatkov V.Ya. Rol` e`strogenchuvstvitel`nyh neyronov arkuatnoy oblasti gipotalamusa v mehanizme osvobozhdeniya lyuteiniziruyushhego gormona. Probl. E`ndokrinol. 1978; 24 (2): 53–9. [Babichev V.N., Ignatkov V.Ia. Role of estrogen-sensitive neurons in the arcuate region of the hypothalamus in the mechanism of luteinizing hormone release. Probl Endokrinol. 1978; 24 (2): 53–9 (in Russian)]
  12. Mora O.A., Sanchez-Criado J.E., Guisado S. Role of the vomeronasal organ on the estral cycle reduction by pheromones in the rat. Rev. Esp. Fisiol. 1985; 41 (3): 305–10.