STABILIZATION OF HUMAN PLATELETS ON THE ADHESIVE SUBSTRATE WITH USE OF TICAGRELOR

DOI: https://doi.org/None

M.S. Makarov, V.B. Khvatov, N.V. Borovkova Emergency Aim Research Institute of N.V. Sklifosovsky, Bol'shaya Sukharevskaya sq, 3, Moscow, 129090, Russian Federation

Introduction. Producing of combined biotransplants with human platelets may be accompanied by high rate of the loss of biological active platelet components. It is suggested to use blockers of platelet activity to fix these cells on adhesive substrate surface. The aim of the study. To assess the ability of human platelet stabilization on the glass and collagen with the use of antiagregant ticagrelor. Methods. Platelet study was performed with morphofunctional platelet rate evaluation, based on vital stained cells analysis with fluorescent microscopy. Results. Presence of ticagrelor in plasma led to the platelet stabilization (anchoring) on substrate cover, saving platelet granules inside cytoplasm, while in samples without ticagrelor the adhesion of platelets was followed by the total degranulation and activation of cells. Conclusion. Ticagrelor is useful for biologically high-grade platelets stabilization on adhesive substrates.
Keywords: 
platelet, granules, ticagrelor, adhesion, stabilization
Список литературы: 
  1. Obolenskiy V.N., Ermolova D.A. Primenenie trombocitarnyh faktorov rosta i kollagenovyh biopreparatov v lechenii bol`nyh s hronicheskimi troficheskimi yazvami razlichnoy e`tiologii. Hirurgiya. 2012; 42 (5): 42–7. [Obolenskij V.N., Ermolova D.A. Application of platelet growth factor and collagen biological products in the treatment of patients with chronic trophic ulcers of various etiology. Hirurgija. 2012; 42 (5): 42–7 (in Russian)]
  2. Marx R.E. Platelet-rich plasma: evidence to support its use. J Oral Maxillofac Surg. 2004; 62 (4): 489–96
  3. Yurchenko M.Yu., Shumskiy A.V. Hirurgicheskoe lechenie parodontita s primeneniem plazmy, obogashhennoy trombocitami. Klin stomatol. 2003; 2: 46–8. [Jurchenko M.Ju., Shumskij A.V. Surgical treatment of parodontit, usin platelet-rich plasma. 2003; 2: 46–8 (in Russian)]
  4. Anitua E., Andia I., Ardanza B., Nurden P., Nurden A.T. Autologousplatelets as a source of proteinsforhealingand tissue regeneration. ThrombHaemost. 2004; 91 (1): 4–15.
  5. Obolenskiy V.N., Ermolova D.A, Makarov M.S., Konyushko O.I., Storozheva M.V., Laberko L.A., Borovkova N.V. Primenenie applikaciy bogatoy trombocitami autoplazmy v lechenii bol`nyh s hronicheskimi ranami razlichnoy e`tiologii. M.: Departament zdravoohraneniya goroda Moskvy, 2013; 16. [Obolenskij V.N., Ermolova D.A, Makarov M.S., Konjushko O.I., Storozheva M.V., Laberko L.A., Borovkova N.V. Application of platelet-rich autoplasma in the treatment of patients with chronic trophic ulcers of various etiology. M.: Moscow Heath Department, 2013; 16 (in Russian)]
  6. Vasil`ev S.A., Vinogradov V.L., Karabudagova Z.K. Struktura i funkcii trombocitov. Gematologiya i transfuziologiya. 2010; 55 (5): 4–9. [Vasil’ev S.A., Vinogradov V.L., Karabudagova Z.K. Structure and function of platelet. Gematologija i transfuziologija. 2010; 55 (5): 4–9 (in Russian)]
  7. Kikuchi L., Park J.Y., Victor C., Davies J.E. Platelet interactions with calcium-phosphate-coated surfaces. Biomaterials. 2005; 26 (26): 5285–95.
  8. Hvatov V.B., Makarov M.S., Borovkova N.V. Morfologicheskaya ocenka adgezivnoy aktivnosti trombocitov s pomoshh`yu vital`nogo okrashivaniya. Klinicheskaya laboratornaya diagnostika. 2013; 7: 58–61. [Khvatov V.B., Makarov M.S., Borovkova N.V. Morphological analysis of platelet adhesive activity with vital staining. Klinicheskaja laboratornaja diagnostika. 2013; 7: 58–61 (in Russian)]
  9. Harrison P., Mumford A. Screening tests of platelet function: update on their appropriate uses for diagnostic testing. Semin Thromb Hemost. 2009; 35: 150–7.
  10. Suggs L.J., West J.L., Mikos A.G. Platelet adhesion on a bioresorbable poly (propylene fumarate-co-ethylene glycol) copolymer. Biomaterials. 1999; 20 (7): 683–90.
  11. Makarov M.S., Kobzeva E.N., Vysochin I.V., Borovkova N.V., Hvatov V.B. Morfofunkcional`nyy analiz trombocitov cheloveka s pomoshh`yu vital`nogo okrashivaniya. Byulleten` e`ksperimental`noy biologii i mediciny. 2013; 9: 388–91. [Makarov M.S., Kobzeva E.N., Vysochin I.V., Borovkova N.V., Khvatov V.B. Morphofunctional human platelet analysis with vital staining. Bjulleten’ jeksperimental’noj biologii i mediciny. 2013; 9: 388–91 (in Russian)]
  12. Makarov M.S., Borovkova N.V., Hvatov V.B., Larin A.G., Kokov L.S. Sposob ocenki chuvstvitel`nosti trombocitov k tikagreloru in vitro. Medicinskiy Alfavit. Sovremennaya laboratoriya. 2013; 2: 34–7. [Makarov M.S., Borovkova N.V., Khvatov V.B., Larin A.G., Kokov L.S.. Assessment of platelets sensitivity to ticagrelor in vitro. Medicinskij Alfavit. Sovremennaja laboratorija. 2013; 2: 34–7 (in Russian)]
  13. Kotova Y.N., Ataullakhanov F.I., Panteleev M.A. Formation of coated platelets is regulated by the dense granule secretion of adenosine 5’diphosphate acting via the P2Y12 receptor. J Thromb Haemost. 2008; 6 (9): 1603–5.
  14. Anderson S.D., Shah N.K., Yim J., Epstein B.J. Efficacy and safety of ticagrelor: a reversible P2Y12 receptor antagonist. Ann Pharmacother. 2010; 44: 524–37.
  15. Marczewski M.M., Postula M., Kosior D. Novel antiplatelet agents in the prevention of cardiovascular complications – focus on ticagrelor. Vasc Health Risk Manag. 2010; 6: 419–29.