INFLUENCE OF NITRIC OXIDE SPATIO TEMPORAL CONCENTRATION GRADIENTS ON CONDITIONS OF HYPOXIA DEVELOPMENT IN TISSUES

DOI: https://doi.org/None

Ya.R. Nartsissov (1,2), S.E. Boronovskiy (1), V.S. Kopylova (1), E.V. Mashkovtseva (1,2) 1 -Institute of cytochemistry and molecular pharmacology, 6th-Radial’naya str., 24/14, Moscow, 115404, Russian Federation 2 -National Research Nuclear University «Moscow Engineering Physics Institute», Kashirskoe Shosse, 31, Moscow, 115409, Russian Federation

Introduction. Nitric oxide is a well-known regulator of different metabolic processes in cells and tissues. Its effect is usually represented as a balanced combination of both mediator and toxic action and the mutual ranges of them are not simply identified. It was previously supposed that inhibition of mitochondrial respiratory chain activity may lead to increase of oxygenated tissue area. The aim of this work is to analyze the mutual influence of spatiotemporal distributions of nitric oxide and oxygen concentrations near the blood vessels. Methods. Estimations of non-steady state gradients of nitric oxide and oxygen were carried out in virtual digital phantoms of rat pial membrane using analytical solutions of the boundary problems for 3D diffusion equation. Results. It was shown that even rapid (100 sec) low amplitude (less than 4,5 nM) nitric oxide gradient forming yields essential increase (more than 2,5 times) of oxygenated area. Conclusion. The observed effect can be considered as an additional compensatory mechanism under early stages of hypoxia and a prohypoxic player under later ones.
Keywords: 
nonsteady-state gradients, nitric oxide, hypoxia, diffusion
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