TO THE QUESTION OF USE OF PARAFFIN BLOCKS WITH MALIGNANT TUMORS FOR IDENTIFICATION OF THE GENETIC IDENTITY OF SAMPLES

DOI: https://doi.org/None

O.V. Dolzhanskiy (1), Yu.I. Pigolkin (2), E.M. Paltseva (1), S.A. Korostylev (3), I.V. Kanivets (3), D.N. Fedorov (1) 1 -B.V. Petrovsky Russian Surgery Research Center, Abrikosovskii per., 2, Moscow, 119991, Russian Federation; 2 -I.M. Sechenov First Moscow State Medical University, Trubetskaya, 8, Moscow, 119991, Russian Federation; 3 -Research Centre of Medical Genetics, Moskvorechie, 1, Moscow, 115478, Russian Federation

On the basis of published data there were assessed criteria of eligibility of paraffin blocks with malignant tumors for the decision of a question of the genetic identity of the samples. In order to prevent potential errors it’s not recommended to use the carcinomas of the gastrointestinal tract, breast and poorly differentiated ovarian tumors for such assays; in comparable samples there should not be recurrent or metastatic tumors, neoplasms after radiation or anticancer drug therapy, material with the presence of microsatellite instability. In doubtful cases, it is important to conduct the immunohistochemical research of MSI, as well as to compare the genotype of the tumor and the blood DNA of patients.
Keywords: 
genetic identity of the material, the tumor paraffin blocks
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