EXPRESSION OF INNATE IMMUNE RECEPTORS AND TRANSCRIPTIONAL REGULATION OF T-HELPER CELLS IN EXPERIMENTAL ILEITIS AND CONDUCT OF THE SIMVASTATIN AND ARIL-1

DOI: https://doi.org/None

A.S. Zherebiatiev, A.M. Kamyshny, V.A. Kamyshnaya Zaporozhye State Medical University, Mayakovsky avenue 26, Zaporozhye, 69035, Ukraine

Introduction. The pathogenesis of inflammatory bowel disease is complex and multifactorial. Studies have led to the current concept that the T-helper cells are components of the adaptive immune response whereas Toll-like receptors, NOD-like-receptors, RIG-I-like receptors represent key mediators of innate host defense in the intestine, and they are involved in maintaining mucosal as well as commensal homeostasis. The aim of the study. We studied the possibility of simvastatin and antagonist of receptors of interleukin-1 for correction of experimental ileitis in rats with afocus on the expression studies of Toll-like receptor 2 (TLR2), Toll-like receptor 4 (TLR4), NOD2, RIG-I and transcription factors T-bet, GATA-3, RORγt and Foxp3 with lymphocytes of small intestine. Methods. Experiments were carried out on male Wistar rats aged of 5–7 months. The immunopositive lymphocytes were determined by using both of a direct and indirect immunofluorescence technique and a monoclonal rat antibody. Results. The study of serial sections of ileum showed that the development of acute and chronic ileitis was accompanied with onedirected tendency on increasing of total number of TLR2+ lymphocytes and decreasing of total number of TLR4+ and Foxp3+ lymphocytes in lymphoid structures of ileum.The administrations of Simvastatin and ARIL-1 in experimental animals during the development of experimental pathology was accompanied by decrease of RORγt+- and T-bet+ lymphocytes,and increasing of total number of Foxp3+ lymphocytes. Conclusion. Simvastatin and antagonist of receptors of interleukin-1 seemed to be beneficial in indomethacin-induced ileitis model in rats through the modulation of the expression of pattern recognition receptors on lymphocytes and balance between different T-helper cell subsets of the small intestine.
Keywords: 
ileitis, pattern recognition receptors, antagonist of receptors of interleukin-1 (ARIL-1), simvastatin
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