SUBPOPULATIONS OF MOBILIZED PERIPHERAL HEMATOPOIETIC STEM CELLS IN CANCER PATIENTS. ASSOCIATION WITH NOSOLOGICAL FORM OF THE DISEASE

DOI: https://doi.org/None

L.Yu. Grivtsova N.N. Blokhin Russian Cancer Research Center, Kashirskoye shosse, 24, Moscow, 115478, Russian Federation

Introduction. Currently there was described a number of subpopulations of haematopoietic stem cells and progenitor cells, important for completeness of the recovery of hematopoiesis and antigens involved in the process of homing, adhesion and mobilization of haematopoietic stem cells. The aim of the study. Characteristics of the subpopulation composition of haematopoietic stem cells and progenitor cells based on membrane immunophenotype to predict the completeness of recovery of hematopoiesis, and the evaluation of the kinetics of mobilization of peripheral blood hematopoietic stem cells. Metods. Subpopulation content of mobilized peripheral blood hematopoietic stem cells (PBHSC) has been studied in 347 patients with oncohaematological malignancies (158 adult and 189 children) and 54 adult donors of allogeneic PBHSC. Results. There is more pronounced effect of mobilization (total number of CD34+ cells) detected in adult patients with multiple myeloma (MM), in children with Ewing's Sarcoma and neuroblastoma, as well as in healthy donors. Relatively to the subpopulation, the effect of mobilization was more pronounced in healthy individuals and children with medulloblastoma and neuroblastoma. Conclusion. According to immunological criteria (CD34+CD38–HLA-DR–Thy-1+) the most completed haematopoiesis recovery takes place after transplantation of donors PBHSCs in adults with Hodgkins lymphoma, children with PNEO, medulloblastoma and neuroblastoma.
Keywords: 
peripheral mobilized hematopoietic stem cells (HSC), subpopulations, oncohaematology, transplantation
Список литературы: 
  1. Greenberg A., Kerr W., Hammer D. Rela-tionship between selectin-mediated rolling of hematopoietic stem and progenitor cells and progression in hematopoietic development. Blood. 2000; 95 (2): 478–86.
  2. Shiftiel S., Lapid K., Kalchenko V., Avigdor A., Goichberg P., Kalinkovich A., Nagler A., Kollet O., Lapidot T.CD45 regulates retention, motility, and numbers of hematopoietic progenitors, and affects osteoclast remodeling of metaphyseal trabecules. J. Exp. Med. 2008; 205 (10): 2381–95.
  3. Shivtiel S., Lapid K., Kalchenko V., Avigdor A., Goichberg P., Kalinkovich A., Nagler A., Kollet O., Lapidot T. CD45 regulates homing and engraftment of immature normal and leukemic human cells in transplanted im-munodeficient mice. Exp. Hematol. 2011; 39 (12): 1161–70.
  4. Driessen R., Johnston H., Nilsson S. Membrane-bound stem cell factor is a key regulator in the initial lodgment of stem cells within the endosteal marrow region. Exp. Hematol. 2003; 31 (12): 1284–91.
  5. Ishikawa F., Livingston A.G., Minamiguchi H., Wingard J.R., Ogawa M. Human cord blood long-term engrafting cells are CD34+CD38–. Leukemia. 2003; 17 (5): 960–4.
  6. Srour E., Tong X., Sung K., Plett P., Rice S., Daggy J., Yiannoutsos C., Abonour R., Or-schell C. Modulation of in vitro proliferation kinetics and primitive hematopoietic po-tential of individual human CD34+CD38–/lo cells in G0. Blood. 2005;105 (8): 3109–16.
  7. Lanza F., Campioni D., Moretti S., Do-minici M., Punturieri M., Focarile E., Pauli S., Dabusti M., Tieghi A., Bassilieri M., Scapoli C., De Angeli C., Galluccio L., Castoldi G. CD34 cell subset and long-term culture colony-forming cells evaluated on both autologous and normal stroma predict long – term hematopoietic engraftment in patients undergoing autologous peripheral blood cell transplantation.. Exp. Hematol. 2001; 29 (12): 1484–93.
  8. Majeti R., Park C.Y., Weissman I.L. Identifica-tion of a hierarchy of multipotent hemat-opoietic progenitors in human cord blood. Cell Stem Cell. 2007; 1: 635–45
  9. Garg S., Madkaikar M., Ghosh K. Inves-tigating cell surface markers on normal hematopoietic stem cells in three different niche conditions. Int. J. Stem Cells. 2013; 6 (2): 129–33.
  10. Humeau L., Bardin F., Maroc C., Alario T., Galindo R., Mannoni P., Chabannon C. Phenotypic, molecular and functional characterisation of human peripheral blood CD34+/Thy-1+ cells. Blood. 1996; 87 (3): 949–55
  11. Dahlberg A., Delaney C., Bernstein I. Ex vivo expansion of human hematopoietic stem and progenitor cells. Blood. 2011; 117 (23): 6083–90.
  12. Seita J., Weissman J.L. Hematopoietic stem cells self-renewal versus differentiation. Wiley Interdisciplinary Reviews. 2010; 2 (6): 640–53.
  13. Kondo M., Wagers A., Manz M., Prohaska S., Scherer D., Beilhack G., Shizuru J., Weiss-man I.L. Biology of hematopoietic stem cells and progenitors: implications for clini-cal applications. Ann. Rev. Immunol. 2003; 21: 759–806.
  14. Schipper L., Brand A., Reniers N., Melief C., Willemze R., Fibbe W.E. Differential matura-tion of megakaryocyte progenitor cells from cord blood and mobilized peripheral blood. Exp. Hematol. 2003; 31 (4): 324–30.