ALTERATION OF HYDROPEROXIDE NON-STEADY STATE GRADIENTS IN THE REOXYGENATION NIDUS EXPOSED TO GLYCINE UNDER EXCITOTOXICITY

DOI: https://doi.org/10.29296/24999490-2018-03-07

Ya.R. Nartsissov, V.S. Kopylova, E.V. Mashkovtseva, S.E. Boronovskiy
Institute of Cytochemistry and Molecular Pharmacology, 6th-Radial’naya str., 24/14, Moscow, 115404, Russian Federation
Е-mail: yarosl@biotic.dol.ru

Introduction. Glycine is well-known to have a positive effect in the treatment of patients suffered from an ischemic stroke. Vasodilatation is one of the main parts of the action of this amino acid at the molecular level. Simultaneously, the increase of oxygen supply after some period of hypoxia/anoxia yields the gain in the generation of reactive oxygen species in the reoxygenation nidus. The aim of this work is to reveal the glycine effects on non-steady state gradients of hydroperoxide concentration in the reoxygenation nidus under the glutamic excitotoxicity. Methods. Alterations in the spatiotemporal distribution of hydroperoxide have been analyzed in the digital phantom of the local area including blood vessels, using analytic solutions of boundary value problems for 3D diffusion equation. Results. The impact of glycine on the hydroperoxide generation due to the glutamate excess was shown to lead to the profound decrease of its concentration gradient in reoxygenation area of the tissue experienced hypoxia. Conclusion. The results of the present study show some features of the glycine regulatory action in the local reoxygenation area under glutamate excitotoxicity.
Keywords: 
non-steady state gradients, glycine, hydrogen peroxide, hypoxia, diffusion