Α-SYNUCLEIN AND AΒ42 PEPTIDE EXPRESSION IN SUBSTANTIA NIGRA AND BUCCAL EPITHELIUM IN PARKINSON DISEASE PATIENTS OF THE DIFFERENT AGE

DOI: https://doi.org/10.29296/24999490-2018-06-06

V.A. Zuev(1), A.S. Diatlova(1, 2), N.S. Linkova(1, 3), V.O. Polyakova(4, 5, 6), I.M. Kvetnoy(4, 5), M.A. Paltsev(7), U.F. Gashimova(8), A.M. Gadzhiev(8) 1-Saint Petersburg Institute of Bioregulation and Gerontology, Dynamo pr., 3, Saint Petersburg, 197110, Russian Federation; 2-Institute of Experimental Medicine, Pavlova Akademika str., 9, Saint Petersburg, 197376, Russian Federation; 3-Peter the Great Saint Petersburg Polytechnic University, Polytechnicheskaya str., 29, Saint Petersburg, 195251, Russian Federation; 4-D.O. Ott Institute of Obstetrics, Gynecology and Reproductology, Mendeleyevskaya line, 3, Saint Petersburg, 199034, Russian Federation; 5-St. Petersburg University, Universitetskaya Emb., 7/9, Saint Petersburg, 199034, Russian Federation; 6-S.M. Kirov Military Medical Academy, Clinicheskaia str., 1, Saint-Petersburg, 194044, Russian Federation; 7-Center of Immunology and Molecular Biomedicine of the M.V. Lomonosov Moscow State University, Leninskye Gory, 1, Moscow, 119234, Russian Federation; 8-Academician A. Karayev Institute of Physiology, Sharuphzade str., 78, Baku, AZ1100, Azerbaijan E-mail: [email protected]

Introduction. The current direction of modern medicine is the development of new methods for non-invasive diagnosis of neurodegenerative diseases, in particular, Parkinson's disease. The goal of the investigation was the comparative analysis of α-synuclein and Аβ42 peptide expression in substantia nigra and buccal epithelium of elderly and old patients with and without Parkinson's disease. Methods. The objects of the morpho-functional investigation were the authopsy material of substantia nigra and biopsy material of buccal epithelium of elderly and old patients. All people were divided into 2 groups: without brain pathology and with Parkinson's disease. α-synuclein and Аβ42 peptide expression were registered by immunohistochemistry and immunocytochemistry methods. The analysis of samples was done with the use of invert confocal microscope Olympus and software Vidеotest Morphology 5.2 for the area and optical density of the molecule expression. Results. α-synuclein and Аβ42 peptide expression in the substantia nigra of elderly and old patients with Parkinson's disease was 23–30 times higher in comparison with persons without neurodegenerative pathology. α-synuclein andАβ42 peptide expression in buccal epithelium of elderly and aged patients with Parkinson's disease was 6–16 times higher in comparison with persons without neurodegenerative pathology. Conclusion. Thus, immunocytochemical study of α-synuclein and Aβ42 peptide expression in buccal epithelium can be an easily available non-invasive method for diagnosing Parkinson's disease in elderly and aged people.
Keywords: 
Parkinson's disease, non-invasive diagnostics, buccal epithelium, α-synuclein, Aβ42 peptide
Список литературы: 
  1. Walker Z., Possin K.L., Boeve B.F., Aarsland D. Lewy body dementias. Lancet. 2015; 386 (10004): 1683–97.
  2. Malek N., Swallow D., Grosset K.A., Anichtchik O., Spillantini M., Grosset D.G. Alpha-synuclein in peripheral tissues and body fluids as a biomarker for Parkinson’s disease – a systematic review. Acta Neurol Scand. 2014; 130 (2): 59–72.
  3. Williams S.M., Schulz P., Sierks M.R. Oligomeric α-synuclein and β-amyloid variants as potential biomarkers for Parkinson’s and Alzheimer’s diseases. Eur J. Neurosci. 2016; 43 (1): 3–16.
  4. Ho C.Y., Troncoso J.C., Knox D., Stark W., Eberhart C.G. Beta-amyloid, phospho-tau and alpha-synuclein deposits similar to those in the brain are not identified in the eyes of Alzheimer’s and Parkinson’s disease patients. Brain Pathol. 2014; 24 (1): 25–32.
  5. Zuev V.A., Lin`kova N.S., Polyakova V.O. Pal`ceva E.M., Kvetnaya T.V., Kukanova E.O. Aβ42 – perspektivnyy neinvazivnyy marker dlya prizhiznennoy diagnostiki bolezni Al`cgeymera. Molekulyarnaya medicina. 2017; 15 (1): 24–7. [Zuev V.A., Linkova N.S., Polyakova V.O., Paltseva E.M., Kvetnaia T.V., Kukanova E.O. Aβ42 – perspective non-invasive marker for live diagnostic of Alzheimer disease. Molecular Medicine. 2017; 15 (1): 24–7 (in Russian)]
  6. Paltsev M.A., Polyakova V.O., Kvetnoy I.M., Anderson G., Kvetnaia T.V., Linkova N.S., Paltseva E.M., Rubino R., De Cosmo S., De Cata S., Mazzoccoli G. Morphofunctional and signaling molecules overlap of the pineal gland and thymus: role and significance in aging. Oncotarget. 2016; 7 (11): 11972–83.
  7. Scherzer C. Searching for biomarkers of Parkinson’s disease. Biomark Med. 2003; 3: 113–4.
  8. Schlossmacher M.G, Mollenhauer B. Biomarker research in Parkinson’s disease: objective measures needed for patient stratification in future cause-directed trials. Biomark Med. 2010; 4: 647–50.
  9. Tsigelny I.F., Crews L., Desplats P., Shaked G.M., Sharikov Y., Mizuno H., Spencer B., Rockenstein E., Trejo M., Platoshyn O., Yuan J. X-J., Masliah E. Mechanisms of hybrid oligomer formation in the pathogenesis of combined Alzheimer’s and Parkinson’s diseases. PLoS One. 2008; 3: e3135.
  10. Badiola N., de Oliveira R.M., Herrera F., Guardia-Laguarta C., Gonçalves S.A., Pera M., Suárez-Calvet M., Clarimon J., Outeiro T.F., Lleó A. Tau enhances alpha-synuclein aggregation and toxicity in cellular models of synucleinopathy. PLoS One. 2011; 6 (10): e26609.
  11. Clinton L.K., Blurton-Jones M., Myczek K., Trojanowski J.Q., LaFerla F.M. Synergistic Interactions between Abeta, tau, and alpha-synuclein: acceleration of neuropathology and cognitive decline. J. Neurosci. 2010; 30 (21): 7281–9.
  12. Masliah E., Rockenstein E., Veinbergs I., Sagara Y., Mallory M., Hashimoto M., Mucke L. beta-amyloid peptides enhance alpha-synuclein accumulation and neuronal deficits in a transgenic mouse model linking Alzheimer’s disease and Parkinson’s disease. Proc Natl Acad Sci USA. 2001; 98 (21): 12245–50.
  13. Pal`cev M.A., Kvetnoy I.M., Polyakova V.O., Konovalov S.S., Litvyakova O.M., Lin`kova N.S., Sevost`yanova N.N., Durnova A.O., Tolibova G.H. Signal`nye molekuly v bukkal`nom e`pitelii: optimizaciya diagnostiki social`no znachimyh zabolevaniy. Molekulyarnaya medicina. 2012; 4: 18–23. [Paltsev M.A., Kvetnoy I.M., Polyakova V.O., Konovalov S.S., Litvyakova O.M., Linkova N.S., Sevostyanova N.N., Durnova A.O., Tolibova G.Kh. Signaling molecules in buccal epithelium: optimization of diagnosis of socially significant diseases. Molecular medicine. 2012; 4: 18–23 (in Russian)]