I.M. Kvetnoy(1, 2), A.O. Drobintseva(1, 3), T.S. Kleimenova(1, 3), V.O. Polyakova(1, 2), К.А. Turkadze(4) 1-D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, Mendeleyevskaya line, 3, Saint Petersburg, 199034, Russian Federation; 2-St.Petersburg University, Universitetskaya emb., 7/9, Saint Petersburg, 199034, Russian Federation; 3-St. Petersburg State Pediatric Medical University, Litovskaya str., 2, Saint Petersburg, 194100, Russian Federation; 4-Sechenov University, Trubetskaya str., 8/2, Moscow, 119991, Russian Federation E-mail: [email protected]

Introduction. Application of placenta hydrolysate in cosmetology opens a new page in anti-aging therapy. Numerous studies have confirmed the antioxidant, anti-inflammatory, anti-pigmental properties of placenta hydrolysate preparations, as well as activation of trophic function and optimization of cellular metabolism. The difference between preparation Melsmon and its analogs is the maximum safety of the drug since it does not include hormones, growth factors, enzymes, it is completely free from toxic substances. Purpose of the study. To evaluate the possibility of using the preparation Malsmon as a regulatory and geroprotective agent for the prevention of skin aging of human fibroblasts during their senescence in vitro Methods. The expression of proteins was compared by immunocytochemistry in cell cultures of human skin fibroblasts at 7th and 14th passages with the administration of preparation Melsmon and in the control group. Results. When analyzing the data of the relative area of expression of the studied markers in the «young culture» of human fibroblasts, an increase in the expression indices of Ki-67, calreticulin, sirtuin-6 and syntaxin under the influence of the Melsmon preparation was found after the 3rd passage. Whereas in the «old culture» on the 14th passage, when placental hydrolysate (Melsmon) was added, activation of the expression of ki-67, sirtuins 1 and 6, to the same level as in the young (control) culture was observed.
Conclusion. The Melsmon preparation has pronounced geroprotective properties with respect to human skin cells, which gives arguments for its further development as a means of stimulating regenerative processes in the skin and preventing its aging

Список литературы: 
  1. Banerjee K.K., Bishayee A., Chatterjee M. Anti-inflammatory effect of human placental extract: a biochemical mechanistic approach. European Review for Medical and Pharmacological Sciences. 1992; 14 (6): 361–6.
  2. Watanabe S., Togashi S.I., Takahashi N. et al. L-tryptophan as an antioxidant in human placenta extract. J. of Nutritional Science and Vitaminology. 2002; 48 (1): 36–9.
  3. Tamura T. A study on the anti-fatigue of the placental extract (Melsmon) and its hormone-like application. Pharmacology and Treatment. 1978; 6 (10): 31–6.
  4. Nordlund J.J., Halder R. An analysis of published and other available data. Dermatologica. 1990; 181 (1): 1–4.
  5. Kim J.H., Choi S.Y., Kim S.O. et al. Effect of local placental extract injection on pigmentary disorders. Abstracts of the Korean J. of Dermatology. 2005; 43 (2): 175.
  6. Kim H.J., Lee J.W., Kim Y.I., Lee M.H. The effect of placental extract on the expression of tyrosinase, TRP-1 and TRP-2 in SK30 melanoma cells. Korean J. of Dermatology. 2003; 41 (12): 1612–8.
  8. Коваленко И.И., Аталян А.В. Опыт применения гидролизата плаценты у женщин с климактерическим синдромом в перименопаузе. Гинекология. 2016; 18 (5): 20–5.
  9. [Kovalenko I.I., Atalyan A.V. Opyt primeneniya gidrolizata placenty u zhenshchin s klimaktericheskim sindromom v perimenopauze. Ginekologiya. 2016; 18 (5): 20–5 (in Russian)]
  10. Измайлова Т.Д. Персонализированные протоколы метаболической коррекции как основа anti-age программ. Инъекционные методы в косметологии. 2016; 1: 24–37.
  11. [Izmaylova T.D. Personalizirovannye protokoly metabolicheskoy korrektsii kak osnova anti-age programm. In’ektsionnye metody v kosmetologii. 2016; 1: 24–37 (in Russian)]
  12. Измайлова Т.Д. Персонализированная ПРП-терапия. Алгоритм подготовки пациента. Инъекционные методы в косметологии. 2016; 2: 19–23.
  13. [Izmaylova T.D. Personalizirovannaya PRP-terapiya. Algoritm podgotovki patsienta. In’ektsionnye metody v kosmetologii. 2016; 2: 19–23 (in Russian)]
  14. Родионов А.Н., Смирнова И.О., Корнишева В.Г. Дерматология для косметологов. Наука и Техника. 2014; 767.
  15. [Rodionov A.N., I.O. Smirnova, V.G. Kornisheva. Dermatologiya dlya kosmetologov. Nauka i Tekhnika 2014; 767 (in Russian)]
  16. Ноздрин В.И., Горелова М.В., Белоусова Т.А. Возрастные изменения эпидермиса кожи волосистой части головы у мужчин. Морфология. 2011; 139 (1): 74–81.
  17. [Nozdrin V.I., Gorelova M.V., Belousova T.A. Vozrastnye izmeneniya ehpidermisa kozhi volosistoj chasti golovy u muzhchin. Morfologiya. 2011; 139 (1): 74–81 (in Russian)]
  18. Ольховский И.А., Горбенко А.С., Столяр М.А. и соавт. Определение мутации в гене кальретикулина у пациентов с подозрением на хронические миелопролиферативные неоплазии. Гематол. и трансфузиол. 2014; 59 (3): 12–5.
  19. [Ol’hovskij I.A., Gorbenko A.S., Stolyar M.A., i soavt. Opredelenie mutacii v gene kal’retikulina u pacientov s podozreniem na hronicheskie mieloproliferativnye neoplazii. Gematol. i transfuziol. 2014; 59 (3): 12–5 (in Russian)]
  20. Yang S., Liu T., Li S., Zhang X. et al. Comparative proteomic analysis of brains of naturally aging mice. Neuroscience. 2008; 154 (3): 1107–20.
  21. Jung J.J., Inamdar S.M., Tiwari A., Choudhury A. Regulation of intracellular membrane trafficking and cell dynamics by syntaxin-6. Biosci. Rep. 2012; 32: 383–91.
  22. Daugas E. et al. Mitochondrio-nuclear translocation of AIF in apoptosis and necrosis. J. Exp. Med. 2000; 192 (4): 571–80.
  23. Sharma S. et al. Yeast Nop2 and Rcm1 methylate C2870 and C2278 of the 25S rRNA, respectively. Nucleic Acids Res. 2013; 41 (19): 9062–76.
  24. Baur J.A. Resveratrol, sirtuins, and the promise of a DR mimetic. Mech Ageing Dev. 2010; 131: 261–9.
  25. McCord R.A., Michishita E., Hong T., Berber E., Boxer L.D., Kusumoto R., Guan S., Shi X., Gozani O., Burlingame A.L., Bohr V.A., Chua K.F. SIRT6 stabilizes DNA-dependent protein kinase at chromatin for DNA double-strand break repair. Aging (Albany NY). 2009; 1 (1): 109–21.
  26. Mao Z., Hine C., Tian X., Van Meter M., Au M., Vaidya A., Seluanov A., Gorbunova V. SIRT6 promotes DNA repair under stress by activating PARP1. Science. 2011; 332 (6036): 1443–6.
  27. Michishita E. et al. Evolutionarily conserved and nonconserved cellular localizations and functions of human SIRT proteins. Mol. Biol. Cell. 2005; 16 (10): 4623–35.
  28. McBurney M.W., Yang X., Jardine K. et al. The mammalian SIR2alpha protein has a role in embryogenesis and gametogenesis. Mol. Cell Biol. 2003; 23 (1): 38–54.