DETERMINATION OF THE DIFFUSION COEFFICIENT FOR THE DRUG RIVANOL IN THE HUMAN BUCCAL MUCOSA IN VITRO

DOI: https://doi.org/10.29296/24999490-2019-06-07

A.A. Selifonov(1, 2), G.N. Naumova(1), V.V. Tuchin(1, 3–5) 1-Saratov State University, Astrakhanskaya str. 83, Saratov, 410012, Russian Federation; 2-Saratov State Medical University, Kazachaya str., 112 B, Saratov, 410012, Russian Federation; 3-Tomsk State University, Lenina Avenue, 36, Tomsk, 634050, Russian Federation; 4-ITMO University, Russian Federation, Kronverksky prospect, 49, Saint Petersburg, 197101, Russian Federation; 5-Institute of Precision Mechanics and Control, Rabochaya str., 24, Saratov, 410028, Russian Federation Е-mail: [email protected]

Introduction. Dyes have long been successfully used in medicine as exogenous chromophores to increase the effectiveness of the interaction of light with biological tissues, as contrast agents and photosensitizers in diagnosis and photodynamic therapy. At present, the current area of medicine is the development of methods for photodynamic therapy, both for the treatment of cancer pathologies and for antibacterial anti-inflammatory procedures, which can effectively influence resistant strains of microorganisms. The aim of the study. The work is devoted to the determination of the effective diffusion coefficient of the preparation Rivanol in the human buccal mucosa in vitro using the method of optical spectroscopy of diffuse reflection with the usage of the model of free diffusion. The active ingredient of the pharmaceutical preparation Rivanol is ethacridine lactate – acridine low toxic dye with antiseptic and photosensitizing properties, the solution of which has absorption bands in the UV/visible range of 320–450 nm, as well as fluorescence in the green. Methods. In this work, the determination of the effective diffusivity of Rivanol in biological tissue was based on measuring the kinetics of changes in the diffuse reflectance spectrum and estimating the effective optical density when fitting experimental data using the equation obtained from the second Fick law and the Beer-Lambert–Bouguer law. Results. For the first time, an effective in vitro diffusion coefficient of Rivanol in human buccal mucous was detected, which on average (n=6) accounted for (2,36±0,73)·10-7 cm2/s. Conclusion. This value correlates with the published data for the diffusion coefficients of other dyes in the skin dermis and the pathologically changed mucosa of the maxillary sinus of a volunteer. The results can be used for the development of modern methods in medicine, combining diagnostic and therapeutic technologies, which are now known under the general name – theranostics, as well as clinicians to develop sound protocols for photodynamic therapy, eliminating the trauma due to laser effects
Keywords: 
effective diffusion coefficient, Rivanol, human buccal mucosa, diffuse reflectance spectrum
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