DETERMINATION OF FcγRIIIb (CD16) EXPRESSION ON THE SURFACE OF BLOOD NEUTROPHILS IN ANTI-PLAGUE VACCINATED PEOPLE

DOI: https://doi.org/10.29296/24999490-2020-02-06

A.L. Kravtsov , S.A. Bugorkova, S.N. Klyueva, V.A. Kozhevnikov, O.M. Kudryavtseva Russian Research Anti-Plague Institute «Microbe», Universitetskaya str, 46, Saratov, 410005, Russian Federation E-mail: [email protected]

Aim. Comparative evaluation of FcγRIIIb (CD16) expression level on the surface of blood neutrophils in vaccinated and unvaccinated people before and after the interaction of сells in vitro with the plague microbe allergen (pestin). Materials and methods. Blood samples of 25 healthy donors not vaccinated against the plague were examined, as well as 40 volunteers who were vaccinated once and several times with the plague vaccine alive, before and during different periods (1, 6 months) after the vaccination. The expression of CD16 on the surface of neutrophil granulocytes was determined by flow cytometry before and after incubation of blood samples for 4 hours with pestin. Results. After vaccination in all subjects there was registered a significant increase in the expression level of the CD16 molecule on the surface of blood neutrophils. After in vitro contact with pestin, the expression level of the FcγRIIIb molecule (CD16) decreased on the surface of neutrophils only in blood samples of people vaccinated against the plague. Conclusion. The change in the neutrophil phenotype from CD16bright to CD16dim, revealed by in vitro interaction with blood pestin grafted against the plague of people, mimics the response of these cells in anaphylactic shock mediating with specific IgG, and apparently represent the result of the functioning of the FcγRIIIb (CD16) as a trigger for netosis.
Keywords: 
FcγRIIIb (CD16) expression, flow cytometry, anti-plague vaccination
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