CELLULAR MICROENVIRONMENT OF UVEAL MELANOMA: CLINICAL AND MORPHOLOGICAL CORRELATIONS AND PREDICTORS OF BAD PROGNOSIS

DOI: https://doi.org/10.29296/24999490-2020-03-04

S.V. Saakyan, G.P. Zakharova, E.B. Myakoshina Helmholtz Moscow Research Centre of Eye Diseases, Sadovaya-Chernogryazskaya str., 14/19, Moscow, 105062, Russian Federation E-mail: [email protected]

Introduction. Uveal melanoma is a malignant neoplasm of neuroectodermal origin, prone to early metastasis. The tumor microenvironment, including «inflammatory cells» and connective tissue cells, affects its progression, angiogenesis and metastasis. Purpose. To carry out clinical and morphological correlations of the cellular microenvironment of uveal melanoma with the detection of predictors of bad prognosis. Methods. 43 enucleated eyes with uveal melanoma (258 histological preparation) of adult patients were examined. Pathological type of tumors: epithelioid cell (n=9), spindle cell type AB (n=15), mixed cell (n=19). Tumor thickness – 4,7±1,3 mm, bazal diameter – 13,5±3,3 mm. T1N0M0 tumors in 6 eyes were detected, which were located juxtapapillary with involvement of the optic nerve head, which required enucleation. The presence and localization of lymphocytes, macrophages, mast cells, plasma cells, myeloid-derived suppressor cells (promyelocytes, neutrophils, eosinophils), fibroblasts and degree of tumor tissue infiltration were analyzed. Statistical methods: Microsoft Excel, Statistica 10.1, Spearman’s rank correlation coefficient – rs. Results. Analyzing the obtained results, we revealed higher correlations of the epithelium-cell type with the presence of macrophages, myeloid- derived suppressor cells (promyelocytes, neutrophils, eosinophils). The high degree of pigmentation correlated mainly with the presence of mast cells, myeloid-derived suppressor cells (promyelocytes, eosinophils), fibroblasts. The high degree of tumor vascularization correlated mainly with the presence of mast cells. At the same time, the moderate correlation between growth of tumor cells by emissaries with mast cells, myeloid-derived suppressor cells (promyelocytes, eosinophils), fibroblasts was noted. Conclusion. Thus, identification of certain cells in uveal melanoma microenvironment may be a predictor of an unfavorable tumor course and may serve as a pretext for the development of accompanying target therapy aimed to eliminating or reprogramming altered immunity cells and connective tissue.
Keywords: 
uveal melanoma, cellular microenvironment
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