DEVELOPMENT OF A PERSONALIZED APPROACH FOR DETERMINING PATHOLOGICAL AREAS IN THE ORAL MUCOSA BASED ON THE DETERMINATION OF THE GINGIVA PERMEABILITY TO METHYLENE BLUE

DOI: https://doi.org/10.29296/24999490-2021-01-07

A.A. Selifonov(1, 2), V.V. Tuchin(1, 3–5) 1-Saratov State University, Astrakhanskaya str,. 83, Saratov, 410012, Russian Federation; 2-Saratov State Medical University, Kazachaya str., 112 B, Saratov, 410012, Russian Federation; 3-Bach Institute of Biochemistry, Research Center of Biotechnology of the RAS, Leninsky prospect, 33, build. 2, Moscow, 119071, Russian Federation; 4-Tomsk State University, Lenin Avenu, 36, Tomsk, 634050, Russian Federation; 5-Institute of Precision Mechanics and Control of the RAS, Rabochaya str., 24, Saratov, 410028, Russian Federation Е-mail: [email protected]

Introduction. Timely diagnosis and a personalized approach to the treatment of diseases is accepted to be one of the most important modern tasks in medicine and in particular, dentistry. The use of pharmaceuticals for the diagnosis and treatment of various diseases is the basis of a new method – theranostics. Biological tissues have a complex multi-component structure; therefore, the diffusion of substances in them is non-linear. The quantitative determination of the permeability of biological membranes for marker agents is an urgent task of medical biophysics. Purpose of the study. In this work, the permeability of the attached pig gingiva is determined concerning an aqueous solution of a thiazine dye, widely used in dentistry – Methylene Blue (MB). Methods. The determination of permeability is based on the calculation of the effective diffusion coefficient of MB into the gingiva tissue in the wavelength range from 200 to 800 nm using diffuse reflection spectroscopy. Using the second Fick law and the Bouguer-Lambert-Beer law, there was obtained an equation to relate the kinetics of the change in effective optical density and the diffusion coefficient of the preparation into biological tissue. Results. For the first time, the permeability of the tissue of the attached pig gingiva was determined for an aqueous MB solution, which was р=(9.91±1.36)·10-7 cm2/s, the effective diffusion coefficient was D=(4.56±0.72)·10-7 cm2/s (n=4) with a thickness of the samples of the gingival mucosa l=0.46±0.09 cm. Conclusion. The results obtained in the work correlate with published data for other biological tissues and can be used in clinical treatment protocols using MB. Since this dye has photosensitizing properties, the results must be taken into account when conducting photodynamic therapy sessions, during both light therapy and diagnostic procedures with the use MB as a marker for pathological areas of the oral mucosa.
Keywords: 
gingival permeability, diffusion coefficient, methylene blue, diffuse reflection spectroscopy
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