FEATURES OF EXPRESSION OF β-AMYLOID IN CEREBRAL ENDOTHELIAL CELLS IN EXPERIMENTAL ALZHEIMER’S DISEASE

DOI: https://doi.org/10.29296/24999490-2021-02-04

Ya.V. Gorina, E.D. Osipova, A.V. Morgun, O.L. Lopatina, E.V. Kharitonova, A.B. Salmina Prof. V.F. Voino-Yasenetsky Krasnoyarsk State Medical University, Partizana Zheleznyaka str., 1, Krasnoyarsk, 660022, Russian Federation E-mail: [email protected]

Introduction. The formation of neurofibrillary plexuses and the accumulation of senile plaques in the parenchyma and cerebral vessels are the key pathological signs of Alzheimer’s disease presenting a neurodegenerative disease. The main component of senile plaques is β-amyloid. The aim of the study. To study the expression of β-amyloid in cells of cerebral endothelium of the hippocampus in experimental Alzheimer’s disease in vivo, to evaluate the production of β-amyloid precursor protein (APP) upon RAGE and CD147 modulation in the cerebral vascular endothelium as part of the BBB model in vitro. Methods. Male mice of lines the B6SLJ-Tg(APPSwFlLon, PSEN1 * M146L * L286V) 6799Vas (genetic model of Alzheimer’s disease) and C57BL / 6 (control group) at the age of 9 months. Male mice of the line the C57BL / 6 at the age of 4 months, which were introduced β-amyloid for modeling Alzheimer’s disease, sham-operated animals were used as a control. The expression of β-amyloid in the hippocampus was studied by immunohistochemistry. Quantitative analysis of APP gene expression in vitro was performed by PCR. Results. The expression of β-amyloid in the endothelium of the hippocampus was significantly (р
Keywords: 
cerebral endothelium, APP, β-amyloid, RAGE, CD147, Alzheimer’s disease
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