Z.I. Gazitaeva(1, 2), A.O. Drobintseva(3), V.O. Polyakova(3), I.M. Kvetnoy(4), A.Yu. Prokopov(5) 1-Institute of Beauty Fijie, 7th Rostovsky Bystreet, 11, Moscow, 119121, Russian Federation; 2-St. Petersburg Institute of Bioregulation and Gerontology Russian Federation, Dynamo Ave. 3, St. Petersburg, 197110, Russian Federation; 3-St. Petersburg State Pediatric Medical University, Litovskaya Str. 2, St. Petersburg, 194100, Russian Federation; 4-Saint-Petersburg Federal Research Institute of Phthisiology and Pulmonology, Healthcare Ministry of Russia: Ligovskiy pr. 2–4, Saint-Petersburg, 191036, Russian Federation; 5ROS-Chimia Ltd, Pervomayskaya str, 15-705, Ekaterinburg, 620000, Russian Federation E-mail: [email protected]

Introduction. The work is devoted to study of new medication Novacutan SBio and Novacutan YBio as potential geroprotectors for biorevitalization of the skin. Klotho was used as a marker – to assess the overall aging of cells; MTH1 – the antioxidant status of cells; AR-1 – U.V. resistance of cells; synthetic activity of cells: elastin and collagen. The aim of the study was to compare the geroprotective properties of Novacutan YBio and Novacutan SBio. Methods. To study the aging of skin cells in culture, we used the method of combined cultivation of fibroblasts and keratinocytes of human skin, and to verify the expression of signaling molecules – aging markers – methods of immunocytochemistry and confocal microscopy. An in vitro experiment was performed to study of biopsies of the skin of patients after applying various therapies using Novacutan preparations. Results. We studied the expression of signaling molecules/markers AP-1, Klotho, Elastin, MTH-1 in samples of “young” and «old» cell cultures of human fibroblasts and keratinocytes when exposed to them with Novacutan YBio and Novacutan SBio. The authors showed a stimulating effect of Novacutan on the expression of markers Klotho, Elastin, MTH-1, and inhibitory effect on AP-1 expression during cell aging in cultures. Under in vitro conditions. Novacutan Y and S preparations were found to activate synthetic processes in the skin. In this case, the combination therapy of laser skin resurfacing with Novacutan biorevitalization courses is the most favorable. Conclusion. These studies allow considering the preparation Novacutan as a drug with pronounced general regulatory and dermatogeroprotective properties
skin aging, biorevitalization, collagen, reparative processes, klotho protein, photoaging

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