Proliferation effect of peptides on orgatypic tissue culture of mammalian and birds

DOI: https://doi.org/10.29296/24999490-2021-05-04

N.I. Chalisova(1, 2), G.A. Rizhak(2), O.M. Ivko(2) 1-Pavlov Institute of Physiology RAS, nab. Makarova 6, S-Petersburg, 199034, Russian Federation; 2-Petersburg Institute of Bioregulation and Gerontology. Petersburg Institute of Bioregulation and Gerontology, pr. Dinamo 3, S-Petersburg, 197110, Russian Federation E-mail: [email protected]

Introduction. The study of the mechanisms of multicellular system regulation and of complex balance between two basic physiologic processes, proliferation and apoptosis, is actual task of the modern molecular biology and medicine. Purpose of the study – the investigation of relative impact of polypeptides and short peptides on the proliferation in the different tissues of mammalian (rats) and birds (chickens). Methods. Organotypic culture of nervous tissue, vessels, cartilages and testis under the effect of short peptides (tetra-and three-peptides) and of complex polypeptides. Results. The proliferation effect and tissue-specific effect of short peptides and polypeptides was established in tissue culture of rats and chick embryons. Conclusion. The base is created for the quick testing of medical preparations on the cellular level by using of organotypic culture of chicken tissues. The parallelism of peptide stimulating effect on the tissues both of mammalian and birds can be suggested that the peptide regulation of different animals species possibly is one the most ancient mechanisms of the live organism evolution.
Keywords: 
short peptides, polypeptides, organotypic tissue culture, proliferation
Список литературы: 
  1. Anisimov V.N., Khavinson V.Kh. Peptide bioregulation of aging: results and prospects. Biogerontology. 2010; 11: 139–49.
  2. Ryzhak A.P., Chalisova N.I., Lin'kova N.S., Halimov R.I., Ryzhak G.A., Zhekalov. Vlijanie polipeptidov na regeneratsiju kletok v kul'ture raznyh tkanej molodyh i staryh krys. Uspehi gerontologii. 2015; 28 (1): 97–103. [Rizhak G.A., Chalisova N.I., Linkova N.S., Khalimov R.I., Ryzhak G.A., Zhekalov A.N. Polypeptide effect on cellular regeneration in different tissue culture of young and old rats. Advances in Gerontology. 2015; 28 (1): 97–103 (In Russian)]
  3. Sinjari B., Diomede F., Khavinson V., Mironova E., Linkova N., Trofimova S., Trubiani O., Caputi S. Short peptides protect oral stem cells from ageing. Stem Cell Reviews and Reports. 2020; 16: 159–66. https://doi.org/ 10.1007/s12015-019-09921-3].
  4. Zhurkovich I.K., Kovrov N.G., Ryzhak G.A., Mironova E.S., Havinson V.H. Identifikatsija korotkih peptidov v sostave polipeptidnyh kompleksov, vydelennyh iz organov zhivotnyh. Uspehi sovremennoj biologii. 2020; 140 (2): 140–8. https://doi.org/10.31857/S004213242002012X [Zhurkovich I.K., Kovrov N.G., Rizhak G.A., Mironova E.S., Khavinson V. Rh. Identification of short peptides in the composition of polypeptide complexes from animal organs. Advances of modern biology. 2020; 140 (2): 140–8. https://doi.org/10.31857/S004213242002012X (In Russian)].
  5. Chalisova N.I., Lin'kova N.S., Nichik T.E., Ryzhak A.P., Dudkov A.V., Ryzhak G.A. Peptidnaja reguljatsija kletochnogo obnovlenija v kul'turah tkanej pochek molodyh i staryh zhivotnyh. Kletochnye tehnologii v biologii i meditsine. 2015; 1: 10–4. [Chalisova N.I., Linkova N., Nichik T.E., Rizhak A.P.,Dudkov A.V. Rizhak G.A. Peptide regulation of the cellular renewal in kidney tissue culture of young and old animals. Cell technologies in biology and medicine. 2015; 1: 10–4 (In Russian)].
  6. Khavinson V., Linkova N., Diatlova A., Trofimova S. Peptide regulation of cell differentiation. Stem Cell Reviews and Reports. 2020; 16: 118–25. https://doi.org/10.1007/s12015-019-09938-8.
  7. Khavinson V., Linkova N., Kozhevnikova E., Trofimova S. EDR Peptide: Possible Mechanism of Gene Expression and Protein Synthesis Regulation Involved in the Pathogenesis of Alzheimer’s Disease. J. Molecules. Special Issue Peptide Therapeutics 2021; 26: 1–16. https://doi.org/10.3390/molecules26010159.
  8. Havinson V.H., Solov'ev A.Ju., Zhilinskij D.V. Shataeva L. K., Vanjushin B. F.. Epigeneticheskie aspekty peptidnoj reguljatsii starenija. Uspehi gerontologii. 2012; 25 (1): 11–6. [Khavinson V. Kh., Solovyev A. Yu., Zhilinski D. V., Shataeva L. K., Vanyushin B. F. Epigenetic aspects of aging peptide regulation. Advances in Gerontology. 2012; 25 (1): 11–6 (In Russian)].
  9. Havinson V.H., Pronjaeva V.E., Lin'kova N.S., Trofimova S.V. Peptidergicheskaja reguljatsija differentsirovki embrional'nyh kletok setchatki. Kletochnye tehnologii v biologii i meditsine. 2013; 1: 57–60. [Khavinson V. Kh., Pronyaeva V.E., Linkova N.S., Trofimova S.V. Peptide regulation of embryonal retinal cell differentiation. Cell technologies in biology and medicine. 2013; 1: 57–60 (In Russian)].
  10. Khavinson V., Linkova N., Diatlova A., Trofimova S. Peptide regulation of cell differentiation. Stem Cell Reviews and Reports. 2020; 16: 118–25. https://doi.org/10.1007/s12015-019-09938-8.].
  11. Havinson V.H., Lopatina N.G., Chalisova N.I., Lin'kova N., Halimov R.I. Vlijanie tripeptida pinealona na formirovanie uslovno-reflektornoj dejatel'nosti u medonosnoj pchely. Fundamental'nye issledovanija. 2015; 2: 491–6. [Khavinson V.H., Lopatina N.G., Chalisova N.I., Linkova N.S., Khalimov R.I. Tripeptide pinealon effect on the generation of conditional reflex function in honey bee apis. Fundamental Researches. 2015; 2: 491–6 (In Russian)].
  12. Savateeva-Popova E.V., Zaharov G.A. Nejroprotektornoe dejstvie peptida EDR v modeli bolezni Parkinsona u Drosophila melanogaster. V kn. Molekuljarno-kletochnye mehanizmy peptidergicheskoj reguljatsii funktsij mozga M.: Nauka, 2018; 141–59. [Savateeva-Popova E.V., Zakharov G.A. Neuroprotector effect of EDR peptide in Parkinson disease in Drosophila melanogaster. In book Molecular-cellular mechanisms of bran function peptidergic regulation. M.: Nauka, 2018; 141–59 (In Russian)].
  13. Khavinson V., Diomede F., Mironova E., Linkova N., Trofimova S., Trubiani O., Caputi S., Sinjari B. AEDG Peptide (Epitalon) Stimulates Gene Expression and Protein Synthesis during Neurogenesis: Possible Epigenetic Mechanism. Molecules. 2020; 25: 1–17. https://doi.org/10.3390/molecules25030609;
  14. Ashapkin V., Khavinson V., Shilovsky G., Linkova N., Vanuyshin B. Gene expression in human mesenchymal stem cell aging cultures: modulation by short peptides. Molecular Biology Reports. 2020; 47 (6): 4323–29. https://doi.org/10.1007/s11033-020-05506-3.
  15. Caputi S., Trubiani O., Sinjari B., Trofimova S., Diomede F., Linkova N., Diatlova A., Khavinson V. Effect of short peptides on neuronal differentiation of stem cells. International Journal of Immunapathology and Farmacology. 2019; 33: 1–12.
  16. Chalisova N. I., Kontsevaja E. A., Vojtsehovskaja M. A., Komashnja. A. V. Reguljatornoe vlijanie kodiruemyh aminokislot na osnovnye kletochnye protsessy molodyh i staryh zhivotnyh. Uspehi gerontologii. 2011; 24 (2):189–97. [Chalisova N.I., Kontzevaya E.A., Voitzekhovskaya M.A., Komashnya A.V.Regulating effect of coded amino acids on basic cellular processes of young and old animals. Advances in Gerontology. 2011; 24 (2): 189–97 (In Russian)].
  17. Havinson V.H.. Chalisova N.I., Lin'kova N.S., Halimov R.I., Nichik T.E. Zavisimost' tkanespetsificheskogo dejstvija peptidov ot ih kolichestvennogo aminokislotnogo sostava. Fundamental'nye issledovanija. 2015; 2: 497–503. [Khavinson V.H., Chalisova N.I., Linkova N.S., Khalimov R.I., Nichik T.E. The dependence of
  18. tissue-specific peptides activity on the number of amino acids in the peptides. Fundamental Researches. 2015; 2: 497–503 (In Russian)]