Analysis of biochemical changes in muscular tissue of rats with hypercholesterolemia combined introduction of simvastatin and coenzym Q10


Z.I. Mikashinovich, I.A. Semenets Rostov State Medical University, Nakhichevansky line, 29, Rostov-on-Don, 344022, Russian Federation E-mail: [email protected]

Introduction. Statins are a group of effective lipid – lowering medications. A common side effect of long-term statin use is myopathy. The mechanism of its development is not fully understood at the molecular level, making it difficult to find effective drugs preventing and correcting muscle damage. According to one hypothesis, the pathogenesis of statin myopathy is based on CoQ deficiency. The aim of the study. To analyze metabolic changes in the muscle tissue in rats with essential hypercholesterolemia when simvastatin and coenzyme were co-administered. Methods. 120 male rats were divided into two groups: the first group was kept on a vivarium diet, and the second group was administered essential hypercholesterolemia. Animals of the experimental group were divided into subgroups: 1 – on a diet without drug administration; 2 – simvastatin, 3 – simvastatin and CoQ10. At the end of the experiment, the concentrations of pyruvic acid, lactic acid, and reduced glutathione, the activity of glutathione reductase, glutathione peroxidase, and succinate dehydrogenase and cytochrome oxidase were determined in animal muscles. Results. In the muscles of animals with hypercholesterolemia, the introduction of simvastatin promotes the transition to anaerobic oxidation. The administration of simvastatin to animals with hypercholesterolemia was shown to induce a depression of the glutathione-dependent link of antioxidant protection and inhibition of respiratory chain enzymes at the level of cytochrome oxidase and succinate dehydrogenase. Against the background of coenzyme administration, multidirectional changes in lactate and pyruvate are registered in favor of the growth of the latter. The level of reduced glutathione tends to decrease compared to the group receiving simvastatin. The activity of glutathione reductase has not changed, and glutathione peroxidase corresponds to the indices of rats with hypercholesterolemia. The activity of succinate dehydrogenase and cytochrome oxidase is recorded at the level of control values. Conclusion. Combined administration of simvastatin and coenzyme is effective for optimizing the mitochondrial respiratory chain, which can be recommended for clinical studies to evaluate the effectiveness of including coenzyme Q10 in complex therapy of myopathy.
statins, myopathy, muscle, ubiquinone, coenzyme Q10

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