INTRODUCTION OF MODIFIED mRNA hTERT, CAUSES SHORT-TERMTELOMERASE ACTIVITY IN HUMAN SKIN FIBROBLASTS

E.B. Dashinimaev, R.R. Faizullin, Zhang Meng, I.A. Muchkaeva, V.V. Terskikh, Y.V. Sukhanov, A.V. Vasiliev
Koltzov Institute of Developmental Biology, Russian Academy of Sciences.

Reprogramming of human cells without genetic modification is increasingly used technology in experimental cell biology. One of these promising methods is the transfection of synthesized mRNA of transcription factors. In this study we synthesized a modified mRNA of the human telomerase reverse transcriptase (hTERT) and it was shown that its introduction into human cells induces a short-term telomerase activity. This strategy can be used in the future to increase proliferative ability of the cell cultures, for different applications.
Keywords: 
telomerase, reprogramming, human skin fibroblasts