BLOOD LYMPHOCYTE APOPTOSIS IN NORM AND PATHOLOGY

With the purpose to determine the content of apoptotic lymphocytes of venous blood in norm and pathology there were examined 40 primary donors of blood, 14 patients with closed abdominal trauma complicated by acute blood loss of at least 30% of circulating blood volume and 49 patients with severe sepsis. The relative amount of lymphocytes in the early and late stages of apoptosis was determined with the use of Annexin V-FITC/7AAD Kit (company Beckman Coulter). It is revealed, that a physiological level of lymphocytes of venous blood, entering in apoptosis, accounts for 2,74±of 0,23%, lymphocytes, died as a result of apoptosis – 0,08±0,01%. Massive blood loss in victims leads to an increase in the concentration of lymphocytes entering into the process of apoptotic death that is caused by hypoxia. In patients with sepsis there is noted an enhancement of lymphocyte apoptosis, which correlates with the severity of the condition of the patients, assessed on a scale of APACHE II, and the content of the cells have already died as a result of apoptosis – with the outcome of the disease. Determination of the concentration of apoptotic lymphocytes in the venous blood in patients with massive blood loss and in patients with sepsis can be used for predicting the course and outcome of the disease.