THE STUDY OF FACTORS AFFECTING THE EFFICIENCY OF LABELING LEUKOCYTESWITH RADIOCOLLOID COMPLEX «99mTs-TEKHNEFIT»

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O.Yu. Kilina (1,2), V.D. Zavadovskaya (1), M.A. Zorkal'tsev (1), V.D. Udodov (1), M.A. Zamyshevskaya (1)
1 -Siberian State Medical University, Tomsk,
2 -Khakassia State University named after Katanov N.F., Abakan

In the paper there are presented results of the study of the mechanisms of accumulation of the radiocolloid preparation in white blood cells. The study included an assessment of the effect of conditions of blood sampling (the diameter of the needle) and incubation conditions (temperature and time regimes) on the viability and functional activity of leukocytes. The studied colloidal complex «Tekhnefit, 99mTc» was established to be accumulated intracellularly, presumably by means of phagocytosis. Series made in the in vitro experiments revealed that the incubation at 37°C for 45–90 minutes provides the most optimal conditions for preserving the viability and functional activity of cells
Keywords: 
colloidal complexes, the migration of labeled white blood cells, the inflammatory process