PREDICTION OF HEMORRHAGIC SYNDROME IN HEMATOLOGICAL PATIENTS ON THE BASISOF FLUORESCENCE ANALYSIS OF MORPHO-FUNCTIONAL STATUS OF PLATELETS

V.B. Khvatov, M.S. Makarov, A.I. Kostin, E.N. Kobzeva, N.V. Borovkova
Sklifosovsky Institute of Emergency Medicine, Moscow

The morphological and functional study of platelets stained with vital fluorochrome dyes has been performed in the blood of 100 donors and 100 hematological patients with thrombocytopenia. In hematologic patients with clinically pronounced hemorrhagic syndrome (PHS), the intensity of stained platelets luminescence was significantly lower than that in patients without PHS and donors. Herewith in PHS hematological patients there was noted a sharp fall in the values of all the morphological and functional parameters of platelets, whereas in hematological patients without HS this fall was less pronounced. There have been proposed indicators for predicting the development of PHS: adhesive activity of platelets, relative level of platelets with the granules at total cell concentration of 2 to 50•109/l and morpho-functional status of platelets. The use of these parameters permits to prognose significant bleeding development due to thrombocytopenia in 90–97,7% of cases.
Keywords: 
platelets, hemorrhagic syndrome, vital staining, luminescence intensity