THE PROFILE OF MICRORNA EXPRESSION IN LIVER IN CHRONIC HEPATITIS C PATIENTS AND THEIR ASSOCIATION WITH CLINICAL PARAMETERS

P.A. Bovt (1), T.V. Vishnevskaya (1), E.A. Nurmukhametova (2), N.P. Blokhina (2), P.G. Deryabin (1),
E.I. Samokhvalov (1), L.V. Kolobukhina (1), I.N. Khlopova (1), E.I. Kelly (2), N.A. Malyshev (2), S.V. Alkhovsky (1)
1-D.I. Ivanovsky Institute of Virology, N.F. Gamaleya Federal Research Center for Epidemiology and Microbiology,
Ministry of Health of Russia, Gamaleya street, 18, Moscow, 123098, Russian Federation;
2-Clinical Hospital of Infectious Diseases №1, Volokolamskoe shosse, 63, Moscow, 125367, Russian Federation

Introduction. Hepatitis C virus (HCV) is a major risk factor for chronic hepatitis, liver cirrhosis and hepatocellular carcinoma. Molecular mechanisms of fibrogenesis are associated with the activation or inhibition of signaling pathways, which are controlling by microRNAs (miRNAs). miRNAs are small non-coding RNAs that regulate gene expression by means of suppressing translation and/or mRNA degradation. Aberrant miRNA expression is related with development of multiple pathological processes, including liver diseases. The aim of the study. Experimental evaluation of miRNA expression in liver biopsies from chronic hepatitis C (CHC) patients and the analysis of the interrelationship with clinical and morphological parameters of the disease. Methods. MiRNA libraries from 12 CHСpatientswere sequenced with the use of the system«MiSeq Illumina» for the genetic analysis. Obtained results were treatedwith the aid of CLC Genomics Workbench 7.0 software. Results. Levels of the expression of 1104 miRNA were detected. The performed statistical analysis revealed the interrelationship between miRNAs expression levels and clinical and morphological data: the degree of histological activity, a stage of fibrosis and HCV genotype. The expression level of miR-21, miR-143, miR-200 and other miRNAscorrelated with the progression of liver fibrosis. Conclusion. The findings indicate to the alteration of the microRNA expression in the progression of liver fibrosis in CHC patients. It can serve as an auxiliary diagnostic and prognostic tool in HCV infection.
Keywords: 
hepatitis C, microRNA, fibrosis, signal pathways