ИЗОТЕРМИЧЕСКАЯ ПЕТЛЕВАЯ АМПЛИФИКАЦИЯ

DOI: https://doi.org/None

А.И. Абуева, Г.А. Ткаченко, кандидат медицинских наук, доцент, О.С. Бондарева ФКУЗ «Волгоградский научно-исследовательский противочумный институт» Роспотребнадзора, Российская Федерация, 400131, Волгоград, ул. Голубинская, д. 7 E-mail: vari2@sprint-v.com.ru

Обзор посвящен методу изотермической петлевой реакции (loop-mediated isothermal amplification – LAMP), который был изобретен в 2000 г. и постепенно становится одной из наиболее популярных изотермических амплификационных технологий. Подробно рассматриваются механизм LAMP-реакции и различные способы детекции ее результата, перечислены преимущества и ограничения метода. Приведена краткая характеристика различных модификаций методики для обнаружения РНК, одновременного поиска нескольких мишеней (мультиплекс-вариант) и количественного определения ДНК. Указаны перспективные направления применения изотермической петлевой реакции амплификации в качестве экспресс-диагностического метода.
Ключевые слова: 
методы амплификации нуклеиновых кислот, изотермическая петлевая амплификация, молекулярная диагностика

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