ASSOCIATION OF MOLECULAR GENETIC VARIANTS OF VITAMIN D RECEPTOR WITH THE GRADE OF INFLAMMATORY CHANGES

DOI: https://doi.org/None

O.V. Guzeeva (1), V.P. Novikova (2), D.A. Kuzmina (1), N.M. Anichkov (1), A.N. Petrovsky (1), I.U. Melnikova (1), V.I. Larionova (1) 1 -North-Western State Medical University named after I.I. Mechnikov, Kirochnaya Str., 41, St. Petersburg, Russian Federation, 191015; 2 -Almazov Federal Heart,Blood and Endocrinology Centre, Akkuratova Str., 2, St. Petersburg, Russian Federation, 197341

Introduction. Although the interrelationship between disorders of the skeletal system with chronic gastroduodenitis (HGD) and infection with Helicobacter pylori (HP) currently has been well-studied in the literature practically there are no data reflecting therelationship of VDR gene polymorphism on the sites of restriction BsmI, TagI, ApaI with Helicobacter pylori infection and morphological characteristics HGD. The aim of our study was to investigate the association of molecular-genetic variants of the gene of the vitamin D receptor with the degree of inflammatory changes of the gastric mucous membrane (GM) in children with HGD in general and with different degrees of HP infection. Methods. There were examined two groups of children: group 1 included 51 children aged 12–17, with morphologically verified diagnosis of HGD and group 2 was consisted of 19 practically healthy children of the same age. Comprehensive gastroenterological examination of the patients of group 1 consisted of fibrogastroduodenoscopy, morphological investigations of the biopsies coolant, identification of HP – by urease test (Helpyl-test), Gas analyzer «HelicoSense» and histological method after cell staining according to RomanovskyGimza. Diagnostics of helikobakteriosis was based on the coincidence of the results of all the methods used. Molecular genetic typing was performed by polymerase chain reaction and with subsequent use of the standard method of restriction fragments length polymorphism (RFLP). There were determined ApaI, TagI, BsmI polymorphisms in the VDR gene. Results. The study found that in healthy children the incidence of T-allele of the VDR gene was 100%, and in patients with HPunassociated CGD – 78,2% (р
Keywords: 
vitamin D receptor gene (gene VDR), chronic gastro-duodenitis, Helicobacter pylori infection, infiltration of the gastric mucosa, children
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