HYALURONIC ACID PREPARATIONS REGULATE THE EXPRESSION OF TYPE I COLLAGEN AND MATRIX METALLOPROTEINASE 9 IN HUMAN SKIN

DOI: https://doi.org/10.29296/24999490-2020-04-09

V.N. Khabarov(1), I.M. Kvetnoy(2–4), N.S. Linkova(2, 5, 6), M.A. Paltsev(7) 1-Hyaluronic acid Research and Development Center, Komsomolskij pr., 38/16–84, Moscow, 119146, Russian Federation; 2-Saint Petersburg Institute of Bioregulation and Gerontology, Dynamo pr., 3, Saint Petersburg, 197110, Russian Federation; 3-St. Petersburg University, Universitetskaya emb., 7/9, Saint Petersburg, 199034, Russian Federation; 4-Saint Petersburg Research Institute of Phthisiopulmonology, Ligovsky prospekt, 2–4, Saint Petersburg, 191036, Russian Federation; 5-Academy of postgraduate education of the Federal Medical Biological Agency of Russia, Volokolamskoe Shosse, 91, Moscow, 125371, Russian Federation; 6-Belgorod State University, Pobedy str. 85, Belgorod, 308015, Russian Federation; 7-Lomonosov Moscow State University, Center of Immunology and Molecular Biomedicine, Faculty of Biology, Leninskie Gory 1, page 12, Moscow, 119234, Russian Federation E-mail: [email protected]

Introduction. The decline in collagen I type synthesis and the gain in matrix metalloproteinases (MMPs) activity are the main characteristics of the molecular skin aging. MMPs are ferments which cleave collagen. The aim of the study was to investigate the influence of hyaluronic acid (HA) in combination with various ions and other additives on collagen I type expression and remodeling of extracellular matrix in skin cells in middle-aged women. Methods. There is a comparative investigation of HA with various biological additives efficiency in 50 women older 40 years after the circular facelift. The investigation of collagen I type expression and MMP9 expression in skin cells was done by immunofluorescence confocal methods with quantitative data analysis by the morphometric method. Results. HA with aurum decreased by 3 times ММР9 expression and increased by 1.4 times collagen I expression in middle-aged women’s skin. Conclusion. Thus, HA with aurum can be recommended as the perspectives geroprotective drug for elasticity, improving skin appearance and its functions during aging.
Keywords: 
hyaluronic acid, skin aging, collagen, MMP
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