IDENTIFICATION OF SHORT PEPTIDES: OPTIMIZATION OF TARGET THERAPEUTIC PROPERTIES OF THE THYMUS MEDICINE

DOI: https://doi.org/10.29296/24999490-2021-03-05

V.Kh. Khavinson(1, 2), I.K. Zhurkovich(3), G.A. Ryzhak(1), E.S. Mironova(1), N.G. Kovrov(3) 1-St. Petersburg Institute of Bioregulation and Gerontology, Dynamo avenue, 3, St. Petersburg, 197110, Russian Federation; 2-Pavlov Institute of Physiology, Makarova emb., 6, St. Petersburg, 199034, Russian Federation; 3-Institute of Toxicology, Federal Medico-Biological Agency, Behtereva st., 1, St. Petersburg, 192019, Russian Federation Е-mail: [email protected]

Introduction. The identification of molecular components contributes to the realization of the targeted properties of multicomponent drugs to study their molecular mechanism and therapeutic targets. The polypeptide complex isolated from cattle thymus (thymalin), and short peptides KE (Lys-Glu), EDP (Glu-Asp-Pro), designed and then synthesized based on the analysis of its amino acid composition, have similar biological effects. The dipeptide EW was previously found in the thymalin medicine. The aim of the study. Identification of short peptides KE and EDP as part of the thymus polypeptide complex. Method. In order to confirm the presence of these peptides in thymalin, the method of ultra-efficient liquid chromatography-mass spectrometry was used. Results. As a result of the study, the short peptides KE and EDP were found to be a part (minor components) of the thymus polypeptide complex (thymalin). Conclusion. The biological and therapeutic activity of the thymalin medicine is due to the effects of the short peptides KE and EDP found in this study, and the dipeptide EW, which was previously discovered in its composition.
Keywords: 
thymus, thymalin, peptides, identification, biological activity
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