The study of beta-glucan immunomodulating effect in the organotypic culture of rat immune tissue

DOI: https://doi.org/10.29296/24999490-2024-04-04

N.I. Chalisova(1, 2), G.A. Ryzhak(2), L.G. Arzhavkina(3), E.V. Mutzina(3)
1-Pavlov Institute of Physiology RAS, Emb. Makarova 6, Saint Petersburg, 199034, Russian Federation;
2-Saint Petersburg Institute of Bioregulation and Gerontology investigation, Dynamo Ave., 3, Saint Petersburg, 197110, Russian Federation;
3-Federal State Budgetary Military Educational Institution of Higher Education “Military Medical Academy named after S.M. Kirov” of the Ministry of Defense of the Russian Federation, str. Academician Lebedev, 6, Saint Petersburg, 194044, Russian Federation

Introduction. An urgent problem in biology and medicine is the identification of biologically active molecules that affect the cellular processes of proliferation and apoptosis in various tissues of the body. Purpose of the study. The purpose of this study was to identify the effect of combinations of immunomodulating beta-glucan with immunodepresive preparation cycklophosphamide in organotypic immune tissue of kidney and thymus of 5-months rats. Material and methods. The method of organotypic cultivation of immune tissues of kidney and thymus was used for rapid screening of the biological activity of the studied beta-glucan. Results. It has been established that by the combined use of beta-glucan and cycklophosphamide the immunodepresive effect is eliminated. Conclusion. The data obtained create the basis for beta-glucan usage as a protector by the immunodepresive action of different substances. The base is created for creation of new drugs for treatment of the oncological and infection deseases.
Keywords: 
beta-glucan cycklophosphamide, organotypic tissue culture, kidney, thymus
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