STUDY OF FUNCTIONAL SUITABILITY TUMOR TROPISM RADIOPHARMACEUTICALS 99MTс-1-THIO-D-GLUCOSE IN THE EXPERIMENT

DOI: https://doi.org/10.29296/24999490-2018-03-11

R.V. Zelchan(1), A.A. Medvedeva(1), I.G. Sinilkin(1), O.D. Bragina(1), V.I. Chernov(1, 2), E.S. Stasyuk(2), E.A. Ilina(2), V.S. Skuridin(2)
1-Tomsk National Research Medical Center, Kooperativny by-street, 5, Tomsk, 634009, Russian Federation;
2-National Research Tomsk Polytechnic University, Tomsk Polytechnic University, Lenina Avenue, 30, Tomsk, 634050, Russian Federation
E-mail: r.zelchan@yandex.ru

Aim of the study: to study the possibility of the use of glucose derivative labeled with 99mTc for cancer imaging in the experiment. Materials and method: the study was performed in cell culture of normal CHO (Chinese hamster ovary cells CHO) and malignant tissues MCF-7 (human breast adenocarcinoma MCF-7). In tubes with cells in an amount of 3 million, there was introduced 25 MBq glucose derivative labeled with 99mTc and incubated for 30 minutes at room temperature. After a centrifugation of the tube with cells the supernatant was removed. Then the measurement of radioactivity in tubes with normal and tumor cells was made. In addition, the study included 40 mice of C57B1/6j lines with tumor lesion of the right femur. For neoplastic lesions model in mice, the rewas used carcinoma Lewis. Following anesthesia, mice were injected intravenously with the 25 MBq glucose derivative labeled with 99mTc. After 15 minutes planar scintigraphy was performedin a matrix of 512×512 pixels for 5 minutes. Results. By means of the measurement of the radioactivity of normal and malignant cells after incubation with the glucose derivative labeled with 99mTc radioactivity of malignant cells was found to be higher. Mean values of radioactivity levels in normal cells were of 0,3±0,15 MBq Mean values of the level of radioactivity in the malignant cells were of 1,07±0,6 MBq. All examined animals were revealed to have increased accumulation of the glucose derivative labeled with 99mTc at the tumor site. The accumulation of the glucose derivative labeled with 99mTc in the tumor was on average twice as high as compared to the symmetric region. The mean value of the index tumor/background ratio was 2,15±0,35. Conclusion. The present study demonstrated glucose derivative labeled with 99mTc to be a prospective radiopharmaceutical for the cancer visualization. In addition, high accumulation of the glucose derivative labeled with 99mTcin the culture of cancer cells and in animals tumor tissue demonstrates tumor tropism of the radiopharmaceutical
Keywords: 
radiopharmaceuticals, glucose derivatives, oncology