TRANSCRIPTOME ANALYSIS IN ONCOLOGY AND DERMATOLOGY

DOI: https://doi.org/10.29296/24999490-2022-01-01

E.Y. Sergeeva, Y.A. Fefelova, Y.V. Bardezkaya Professor V.F. Voino-Yasenetsky Krasnoyarsk State Medical University, Partizana Zheleznyaka str., 1, Krasnoyarsk, 660022, Russian Federation

Personalized therapy allows to reveal subtypes of diseases with similar symptoms but various molecular mechanisms of development. Different -omics (genomics, epigenomics, transcriptomics, proteomics ets) are used to divide the diseases into subtypes. Transcriptomics is the investigation of whole RNA profile coding by the genome of single cell in specific time period or under specific circumstances. Transcriptome is all RNA transcripts produced by the genome in some time. The aim of the review is to summarize the modern data on perspective methods of investigation – microarray and next generation sequencing (NGS), to disclose the advantages and peculiarities of every method and the use in dermatology and oncology. Material and methods. The materials are the results of the investigations on the theme of russian and foreign researchers and ours published data over the past 13 years, from 2007 till 2020. The data was obtained from biomedical on-line databases PubMed, EMBASE, MedLine. Results. Modern data on microarray and next generation sequencing in the context of transcriptome investigations are summarized in the article. The choice of method is based on the peculiarities and tasks of the investigation. Recently transcriptome investigations are used in many medicine fields including oncology and dermatology that promotes the development of personalized therapy and precise prognosis of diseases. Conclusion. Transcriptome investigations allow to assess the alterations of gene expression profile after the influence of etiologic factors that extends the understanding of diseases pathogenesis and leads to the increased effectivity of therapy
Keywords: 
transcriptome, microarray, sequencing, personalized therapy
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