EFFECT OF DIPEPTIDES ON CELLULAR GROWTH IN ORGANOTYPIC CULTURE OF TISSUES FROM YOUNG AND OLD RATS

DOI: https://doi.org/10.29296/24999490-2022-04-09

N.I. Chalisova(1, 2), G.A. Ryzhak(1), V.Kh. Khavinson(1, 2)
1-Saint Petersburg Institute of Bioregulation and Gerontology, Dynamo pr., 3, Saint-Petersburg, 197110, Russian Federation;
2-Pavlov Institute of Physiology, Russian Academy of Sciences, Makarova emb., 6, Saint-Petersburg, 199034, Russian Federation

Dipeptides are physiological products of protein degradation and informational molecules. Asp-Pro, Asp-Ala, Glu-Ala peptides have geroprotective, reparative properties. The aim of the work is to evaluate the effect of the Asp-Pro, Asp-Ala и Glu-Ala peptides on the development of organotypic tissue culture (bronchi, vessels, brain subcortical structures)in young and old rats. Material and methods. Tissues for organotypic cultivation of bronchi, vessels, brain subcortical structures were obtained from young (2–3 months) and old (18–20 months) Wistar rats. Results. Asp-Pro peptide stimulated cell growth in bronchi tissues of young and old animals. Asp-Ala peptide activated cell proliferation in vessels tissues of young and old rats. Glu-Ala peptide had a stimulating effect on the brain subcortical structures tissue of young and old rats. Conclusion. Asp-Pro, Asp-Ala, Glu-Ala peptides have effects on corresponding tissues obtained from animals of different ages. This indicates the possibility of base formation for working of preparations, including the geroprotective, perfecting the functional activity of bronchi, vessels, brain subcortical structures.
Keywords: 
dipeptides, organotypic cell tissue, cellular growth, aging
Список литературы: 
  1. Журкович И.К., Ковров Н.Г., Рыжак Г.А., Миронова Е.С., Хавинсон В.Х. Идентификация коротких пептидов в составе полипептидных комплексов, выделенных из органов животных. Успехи современной биологии. 2020; 140 (2): 140–8. https://doi.org/10.31857/S004213242002012X. / [Zhurkovich I.K., Kovrov N.G., Ryzhak G.A., Mironova E.S., Khavinson V.Kh. Identification of short peptides in the composition of polypeptide complexes isolated from animal organs. Biology Bulletin Review. 2020; 140 (2): 140–8. https://doi.org/10.31857/S004213242002012X (In Russian)].
  2. Khavinson V., Ilina A., Kraskovskaya N., Linkova N., Kolchina N., Mironova E., Erofeev А., Petukhov M. Neuroprotective Effects of Tripeptides – Epigenetic Regulators in Mouse Model of Alzheimer’s Disease. Pharmaceuticals. Special Issue Epigenetic Drugs. 2021; 14 (6): 515. https://doi.org/10.3390/ph14060515.
  3. Avolio F., Martinotti S., Khavinson V.Kh., Esposito J.E., Giambuzzi G., Marino A., Mironova E., Pulcini R., Robuffo I., Bologna G., Simeone P., Lanuti P., Guarnieri S., Trofimova S., Procopio A.D., Toniato E. Peptides Regulating Proliferative Activity and Inflammatory Pathways in the Monocyte/Macrophage THP-1 Cell Line International J. of Molecular Sciences 2022; 23: 3607–9.
  4. Chalisova N.I. Effect of synthesized dipeptide L-Leu-Lys on cell proliferation and apoptosis in organotypic tissue culture from rat spleen. Applied Cell Biology. 2015; 4 (2): 27–31.
  5. Хавинсон В.Х., Рыжак Г.А., Линькова Н.С., Миронова Е.С. Пептид, обладающий бронхопротекторным действием, фармацевтическая композиция на его основе и способ ее применения. Патент РФ RU 2 767 532 C1. Бюл. №8 от 17.03.2022. [Khavinson V., Ryzhak G.A., Linkova N.S., Mironova E.S. Peptide possessed a bronchitropic action, pharmaceutic composition on its basis and its using method. Patent RF RU 2 767 532 C1. Bul. №8 from 17.03.2022 (In Russian)].
  6. Хавинсон В.Х., Рыжак Г.А., Линькова Н.С., Миронова Е.С. Пептид, улучшающий функциональные свойства эндотелия сосудов, фармацевтическая композиция на его основе и способ ее применения. Патент РФ RU 2 769 051 C1. Бюл. №10 от 28.03.2022. [Khavinson V., Ryzhak G.A., Linkova N.S., Mironova E.S. Peptide perfect the vessel endothelium functional characters, pharmaceutic composition on its basis and its using method. Patent RF RU 2 769 051 C1 Bul. №10 from 28.03.2022 (In Russian)].
  7. Хавинсон В.Х., Рыжак Г.А., Линькова Н.С., Миронова Е.С. Пептид, обладающий нейростимулирующей активностью и восстанавливающий способность к обучению и формированию памяти, фармацевтическая композиция на его основе и способ ее применения. Патент РФ RU 2 768 475 C1. Бюл. №9 от 24.03.2022. [Khavinson V., Ryzhak G.A., Linkova N.S, Mironova E.S. Peptide possessed a neuritstimulating action and restoring ability to learning and the memory formation, pharmaceutic composition on its basis and its using method. Patent RF RU 2 769 051 C1 Bul. №9 from 24.03.2022 (In Russian)].
  8. Ilina A., Khavinson V., Linkova N., Petukhov M. 4,5-Neuroepigenetic Mechanisms of Action of Ultrashort Peptides in Alzheimer’s Disease. Int. J. Mol. Sci. 2022; 23: 4259. https://doi.org/10.3390/ijms23084259
  9. Хавинсон В.Х. Лекарственные пептидные препараты: прошлое, настоящее, будущее. Клиническая медицина. 2020; 98 (3): 165–77. https://doi.org/10.30629/0023-2149-2020-98-3-165-177. [Khavinson V.Kh. Medicinal peptide drugs: past, present, future. Clinical Medicine. 2020; 98 (3): 165–77. https://doi.org/10.30629/0023-2149-2020-98-3-165-177 (In Russian)].
  10. Fridman N.V., Linkova N.S., Kozhevnikova, E.O., Gutop E.O. Khavinson V.Kh. Comparison of the effects of KE and AED peptides on functional activity of human skin fibroblasts during their replicative aging. Bull. Exp. Biol. Med. 2020; (3): 154–7. https://doi.org/0.47056/1814-3490-2020-3-197-201.