IGHT JUNCTION IN THE MAMMARY GLAND EPITHELIUM: POSSIBLE ROLE IN THE DEVELOPMENT OF ITS DYSFUNCTION

DOI: https://doi.org/None

N.M. Kruglova, A.G. Markov Saint Petersburg State University, Universitetskaya nab., 7/9, St. Petersburg, Russian Federation, 199034

Introduction. Tissue barriers are the essential part of the compartmentalization in different organs. Tight junctions play a key role in paracellular transport. The selective permeability of tight junctions depends on expression of the claudin family of tight junction transmembrane proteins. The aim of the study. Here the presence of claudins in mouse mammary gland alveolar epithelium was studied. Methods. Expression and localization of claudins was analyzed with the use of light and electron microscopy, Western blots and confocal laserscanning microscopy. Results. The data of electron microscopy and occludin application as a protein marker showed the presence of well developed tight junctions net localized between alveolar epithelial cells. Claudin-1, -2, -3, -4, -5, -7, -8, -12, -15, -16,-17 and occludin were revealed in mammary gland tissue by Western-blot analysis. In particular, occludin was identified as a protein with the molecular weight of 60-65 kDa; molecular weight of claudin-1, -2, -3, -7, -8, -17 was 22 kDa; claudin-4, -5 and -15 – 24 kDa; claudin-11 and -12 – 26 kDa. Confocal laser-scanning immunofluorescent microscopy showed claudins to be located in tight junctions of alveolar epithelium. However, the distribution of singular claudins in mammary gland tissue is various. Claudin-1, -4, -12 and -16 were supposed to be constitutive components of tight junctions, and oppositely claudin-2, -5, -7, -8, -15 were shown to be the inducible unit of the structure. Conclusions. The functional variety of claudins in tight junctions of mammary gland alveolar epithelium may serve as a molecular basis for the maintaining of alveolar integrity as well as for selective paracellular transport during secretion.
Keywords: 
mammary gland, tight junction proteins, claudins, occludin
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