MOLECULAR ASPECTS OF IN VITRO VESSELS AGING

DOI: https://doi.org/None

K.L. Kozlov (1), V.M. Soldatov (1), V.O. Polyakova (2), N.S. Linkova (1,3), A.O. Durnova (2), D.S. Medvedev (1) 1 -Saint Petersburg Institute of Bioregulation and Gerontology, Dunamo pr, 3, Saint Petersburg, 197110, Russian Federation; 2 -D.O. Ott Research Institute of Obstetrics and Gynecology; Mendeleyevskaya line, 3, Saint Petersburg, 199034, Russian Federation; 3 -Saint Petersburg State Polytechnical University, Polytechnicheskaya str. 29, Saint Petersburg, 195251, Russian Federation

Introduction. Understanding of molecular and cellular mechanisms of vessels aging is the base of new approach creating of drugs for cardio-vascular pathology treatment. The goal of this work is the investigation of molecular and cellular mechanisms of vessels aging in cell cultures. Methods. It was used the method of primary dissociate cell cultures and the method of immunofluorescence confocal laser microscopy. We have applied cells of the 3rd, 7th and 14th passages as young, mature and old cells for cell aging investigation. Results. The curve cell growth has shown that the number of cells in old culture in comparison with young and mature cell cultures was decreased more than in 2 times durind 2-weeks observation. The endothelial cell aging on the molecular level was characterized as increasing of p16 protein expression, which is corresponded to cell cycle bloking, in 5 times in comparison with the young cell culture. Moreover, the expression of other markers of cell senescence (р21, р53) in old cell cultures increased only by 35–79% in comparison with young cell cultures. Conclusion. Aging of vessel cells in vitro has realized to a greater extent as a signal cascade, which is activated by p16 protein, and in a minor extent as p21 and p53 proteins signal cascade. The potential target for vaso- and antiage-protective drugs can be p16 protein and cyclin-dependent kinase CDK4/CDK6.
Keywords: 
vessels, cell aging, cell culture, confocal microscopy
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