INFLAMMATORY MARKERS OF CORONARY ATHEROSCLEROSIS

DOI: https://doi.org/None

E.V. Kashtanova (1), Ya.V. Polonskaya (1), А.V. Kurguzov (2), O.V. Kamenskaya (2), Yu.I. Ragino (1), A.M. Chernyavsky (2) 1 -Research Institute of Therapy and Preventive Medicine, B. Bogatkova str., 175/1, Novosibirsk, 630089, Russian Federation; 2 -E.N. Meshalkin Institute of Circulatory Diseases, Rechkunovskaya str., 15, Novosibirsk, 630055, Russian Federation

Introduction. Currently in the world there are conducted numerous studies on the search for biomarkers associated with increased risk of coronary heart disease (CHD). The aim of the study. The study of the levels of such inflammatory biomarkers as the tumor necrosis factor (TNFα), interleukin (IL1β, IL6, IL8), C-reactive protein (СRP) and homocysteine and sulfated glycosaminoglycans (sGAG) in the blood of men with coronary atherosclerosis and without coronary artery disease. Methods. Age of men included in the study, was 46–79 years. The blood was collected once in the morning on an empty stomach. The levels of inflammatory markers were determined by enzyme-linked immunosorbent assay, sGAG – method-binding dye Allenby blue in the microplate format. Statistical processing was performed in SPSS for Windows. The criterion of reliability was the level of p
Keywords: 
inflammatory biomarkers, atherosclerosis, sulfated of glycosaminoglycan
Список литературы: 
  1. Boren J., Gustaffson M., Skalen K., Flood C., Innerarity T.L. Role of extracellular retention of low density lipoproteins in atherosclerosis. Curr. Opin. Lipidol. 2000; 1: 451–6.
  2. Leta G.C., Mourao P.A., Tovar A.N. Human venous and arterial glycosaminoglycans have similar affinity for plasma low-density lipoproteins. Biochim. Biophys. Acta. 2002; 1586: 243–53.
  3. Fasio S., Babaev V.R., Muttay A.B., Hasty A.H., Carter K.J., Gleaves L.A., Atkinson J.B., Linton M.F. Increased atherosclerosis in mice reconstituted with apolipoprotein E null macrophages. Proc. Natl. Acad. Sci. USA. 1997; 94: 4647–52.
  4. Korobov G.A., Sazonova M.A., Orehov A.N., Sobenin I.A. Regulyatornoe deystvie interleykinov pri ateroskleroze. «Aktual`nye problemy sovremennoy nauki» c materialami Vos`moy Mezhdunarodnoy Telekonferencii (28–31 maya 2012 g.). [Korobov G.A., Sazonova M.A., Orehov A.N., Sobenin I.A. The regulatory effect of interleukins in atherosclerosis. «Pressing problems of a modern science» with the materials of the Eighth International Conference (28–31 may 2012) (in Russian)]
  5. Nasonov E.L. Immunologicheskie markery ateroskleroza. Ter. arhiv. 2002; 5: 80. [Nasonov E.L. Immunological markers of atherosclerosis. Ther. archive, 2002; 5: 80 (in Russian)]
  6. Titov V.N. Rol` makrofagov v stanovlenii vospaleniya, deystvie interleykina-1, interleykina-6 i aktivnost` gipotolamo-gipofizarnoy sistemy. Klin. lab. diagn. 2003; 12: 3–11. [Titov V.N. The role of macrophages in the development of inflammation, the action of interleukin-1, interleukin-6 and the activity of hypothalamo-pituitary system. Clin. Lab. Diag. 2003; 12: 3–11 (in Russian)]
  7. Maseri A., Cianflone D. Inflammation in acute coronary syndromes. Eur. Heart J. 2002; 4 (Suppl. B): 8–13.
  8. Yudkin J.S., Stehouwer C.D., Emeis J.J., Coppack S.W. C-reactive protein in healthy subjects: associations with obesity, insulin resistance, and endothelial dysfunction – a potential role for cytokines originating from adipose tissue? Arterioscler. Thromb. Vascular. Biol. 1999; 19: 972–8.
  9. Woods A., Brull D.J., Humphries S.E., Montgomery H.E. Genetics of inflammation and risk of coronary artery disease: the central role of interleukin-6. Eur. Heart J. 2000; 21: 1574–83.
  10. Zhiburt E.B., Serebryanaya N.B. Citokiny v krovetvorenii, immunogeneze i vospalenii. Fiziologiya. 1996; 3: 15–20. [Zhiburt E.B., Serebrjanaja N.B. Cytokines in the blood, immunogenesis and inflammation. Physiology. 1996; 3: 15–20 (in Russian)]
  11. Nikitin Yu.P., Motina O.V., Ragino Yu.I., Simonova G.I., Ivanova M.V., Shherbakova L.V., Malyutina S.K. Gipergomocisteinemiya v muzhskoy populyacii Novosibirska. Ross. kardiol. Zhurnal. 2007; 4 (66): 63–9. [Nikitin Ju.P., Motina O.V., Ragino Ju.I., Simonova G.I., Ivanova M.V., Shherbakova L.V., Maljutina S.K. Hyperhomocysteinemia in the male population of Novosibirsk. Russian Journal of Sardiology. 2007; 4 (66): 63–9 (in Russian)]
  12. Cherepahin D.I., Bazylev V.V., Evtyushkin I.A., Suchkov S.V, Bogopol`skaya O.M., Charchyan E`.R., Belov Yu.V., Pal`cev M.A. Sovremennye markery v diagnostike ateroskleroza. Kardiologiya i serdechno sosudistaya hirurgiya. 2012; 3: 26–9. [Cherepahin D.I., Bazylev V.V., Evtjushkin I.A., Suchkov S.V, Bogopol’skaja O.M., Charchjan Je.R., Belov Ju.V., Pal’cev M.A. Modern markers in the diagnosis of atherosclerosis. Cardiology and cardiovascular surgery. 2012; 3: 26–9 (in Russian)]
  13. Nozadze D.N., Burmistenko O.S., Semenova A.E., Sergienko I.V., Balahonova T.V., Vlasik T.N. Instrumental`nye i laboratornye metody v vyyavlenii nestabil`nyh ateroskleroticheskih blyashek. Ateroskleroz i dislipidemii. 2013; 3: 4–10. [Nozadze D.N., Burmistenko O.S., Semjonova A.E., Sergienko I.V., Balahonova T.V., Vlasik T.N. Instrumental and laboratory methods in detecting unstable atherosclerotic plaques. J. atherosclerosis and dyslipidaemias. 2013; 3: 4–10 (in Russian)]
  14. Adachi T., Hori S., Miyazaki K., Nakagawa M., Inoue S., Ohnishi Y., Nakazawa H., Aikawa N., Ogawa S. Inhibition of nitric oxide synthesis aggravates myocardial ischemia in hemorrhagic shock in constant pressure model. Shock. 1998; 9 (3): 204–9.
  15. Blankenberg S., Rupprecht H.J. Cytomegalovirus infection with interleukin-6 response predicts cardiac mortality in patients with coronary disease. Circulation. 2001; 103: 2915–21.
  16. Ragino Yu.I., Chernyavskiy A.M., Volkov A.M., Volkova I.I., Voevoda M.I. Faktory i mehanizmy razvitiya koronarnogo ateroskleroza. Novosibirsk: Nauka, 2011. [Ragino Ju.I., Chernjavskij A.M., Volkov A.M., Volkova I.I., Voevoda M.I. The factors and mechanisms of development of coronary atherosclerosis. Novosibirsk: Nauka, 2011 (in Russian)]
  17. Wald D.S., Law M.R., Wald N.J. and Morris J.K. Serum homocystein and coronary heart disease. Coronary heart epidemiology. Oxford: University press. 2005; 16: 239–50.
  18. Ragino Yu.I., Chernyavskiy A.M., Tihonov A.V., Cymbal S.Yu., Polonskaya Ya.V., Semaeva E.V., Ivanova M.V., Voevoda M.I. Urovni lipidnyh i nelipidnyh biomarkerov v krovi u muzhchin s koronarnym aterosklerozom v Novosibirske. Rossiyskiy kardiologicheskiy zhurnal. 2009; 2: 31–7. [Ragino Ju.I., Chernjavskij A.M., Tihonov A.V., Cymbal S.Ju., Polonskaja Ja.V., Semaeva E.V., Ivanova M.V., Voevoda M.I. Urovni lipid and non-lipid biomarkers in the blood of men with coronary atherosclerosis in Novosibirsk. Russian J. of Sardiology. 2009; 2: 31–7 (in Russian)]
  19. Materialy Mezhdunarodnoy Federacii klinicheskoy himii i laboratornoy mediciny Insbruk, Avstriya, 7–11 iyunya 2009 g. Klin-lab konsilium. 2009; 5: 73–9. [Materials of the International Federation of clinical chemistry and laboratory medicine Innsbruck, Austria, from 7 to 11 June 2009. Clin. and lab. cons. 2009; 5: 73–9 (in Russian)]
  20. Babaeva A.R., Davydov S.I., Emel`yanova A.L., Tarasov A.A. Immunologicheskie metody ob``ektivizacii diagnoza ostrogo koronarnogo sindroma. Metodicheskie rekomendacii dlya vrachey. Volgograd, 2007. [Babaeva A.R., Davydov S.I., Emel’janova A.L., Tarasov A.A. Immunological methods objectification diagnosis of acute coronary syndrome. Methodical recommendations for doctors. Volgograd, 2007 (in Russian)]