CHARACTERISTICS OF DNA-METHYLATION IN UTERINE FIBROIDS

DOI: https://doi.org/None

Kiselev V.I., Radzinsky V.E., Shalaev O.N., Eseneeva F.M., Poloznikov A.A., Babkina I.O., Salimova L.Y., Karibova S.I.

Introduction authors made an attempt to demonstrate the role of DNA-methylation in pathogenesis of uterine fibroids. The epigenetic aspects based on personal investigations and literary data have been reviewed. The research underlined a perspective of investigation of epigenetic mechanisms and their future application. Study objective was to evaluate the pathogenetic relevance of DNA-methylation of ESR1α, PgR-B and WNT–inhibitory factor1 in the development and pathogenesis of uterine fibroids. Methods the study included 30 female patients aged 35–52 years with uterine fibroids who were candidates for consevative miomectomy or hysterectomy. The samples from the myoma nodules and satellite myometrium from the same patients were undergone to DNA extraction, «touchdown» PCR- amplification , sequencingand statistic analyses of results has been performed. Study results: hypomethylation in promotor region of the gene both of the ESR1α receptor and PgR-B receptor and hypermethylation in promotor region of the WIF1 gene have been found. Conclusion theinvestigation of DNA-methylation status of genes ESR1 and PgR-B in this sampling is irrelevant, but hypermethylation of WIF1 that activate WNT-signal pathway, can play the key role in the appearance of uterine fibroids and requires further research.
Keywords: 
uterine fibroids, epigenetics, DNA-methylation, ESR1α, PgR-B, WIF1

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