ТНЕ АСTIVITIES OF PROTEASOMES AND CALPAINS IN THE STOMACH CANCER AND MUCOSA IN FOCAL ATROPHIC GASTRITIS

DOI: https://doi.org/10.29296/24999490-2018-01-06

E.V. Ivanova, I.V. Kondakova, O.V. Cheremisina, S.G. Afanasiev Tomsk Cancer Research Institute, Kooperativny bystreet, 5, Tomsk, 634050, Russian Federation Е-mail: [email protected]

Introduction. Aproblem of finding of new markers of the development and progression of gastric cancer is actual. In this context, investigations on the role of proteolytic system sin this pathologyare gaining in importance. The aim of the study. The study of the functioning ofproteasomesandcalpainsingastric tumorscompared tomucosainthe antralfocalatrophic gastritis. Methods. The chymotrypsin-likeactivity ofthe total proteasome pool, activity of 26Spoolandalternative forms of the proteasomes, as well as the generalcalpainactivity were detectedinthe clarifiedhomogenates of the tumorandintacttissueof the stomachand in mucosa in antralfocalatrophic gastritis in the reaction of hydrolysisfluorogenicoligopeptide Suc-LLVY-AMC using specificinhibitors. Results. The study has showna significantincrease in thechymotrypsin-like activity of the totalpool ofproteasome, activities of 26S-and20S-proteasome poolsandtotal activity of calpainsingastric tumorscompared to the intacttissue and mucosa inantralfocalatrophic gastritis. Moreover, the study revealedsignificant changes in the both totalproteasome andcalpainactivity dependingon the presence of lymph nodemetastases of gastric cancer. Conclusion. Thus, the identifiedchanges in proteasome and calpainsystemsin gastric cancerlead to the conclusionof the irsignificant role in the development and progressionof these disease.
Keywords: 
proteasome, 26S pool and 20S poolproteasome, calpains, stomach cancer, antralfocal atrophic gastritis
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