PROGNOSTIC SIGNIFICANCE EXPRESSION OF MRNA TOP1, TOP2Α, TUBΒ3, TYMS, ABCC5 AND GSTP1 GENES IN PATIENTS WITH NON-SMALL CELL LUNG CANCER

DOI: https://doi.org/10.29296/24999490-2018-04-04

I.V. Deryusheva, M.M. Tsyganov, E.O. Rodionov, L.A. Efteev, M.K. Ibragimova, S.V. Miller, S.A. Tuzikov, N.V. Litviakov Саnсеr Rеsеаrсh Institute, Tomsk National Research Medical Center of Russian Academy of Sciences, Kooperativnystr, 5, Tomsk, Russian Federation,634050 E-mail: [email protected]

Introduction. Despite the continuous improvement of the surgical method of treatment of patients with non-small cell lung cancer (NSCLC), there is no noticeable tendency to improve survival data. This 5-year index does not exceed 15–25%. In this regard, it is important to search for new prognostic factors and markers that would provide a high probability to predict the outcome of the disease. The aim of the study was to evaluate the prognostic significance of the level of expression of chemosensitivity genes in NSCLC patients. Materials and methods. The study included 43 NSCLC patients at the stage IIA-IIIB. RNA was isolated from the operating samples of normal and tumor lung tissues after neoadjuvant chemotherapy (NAC). The level of expression of the chemosensitivity genes TOP1, TOP2α, TYMS, ABCC5, TUBβ3, GSTP1 was evaluated by reverse-transcriptase quantitative real-time PCR (RT-qPCR). Results. Analysis of survival curves by the Kaplan-Mayer method allowed to establish significant differences in the parameters of total and disease-free survival in groups of patients with different levels of expression of TUBβ3, TOP2α and GSTP1. The rates of total and disease-free survival in patients with TUBβ3 expression are less than 0,3 (p=0,006 and p=0,02, respectively). The presence in the residual tumor of GSTP1 expression above 0,9 is associated with low disease-free survival (p=0,04). Expression of the TOP2α gene less than 0,1 is statistically associated with a good prognosis of total disease-free survival (p
Keywords: 
non-small cell lung cancer, chemosensitivity genes, mRNA expression, total disease-free survival
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