QUANTITATIVE DISTRIBUTION OF APOLIPOPROTEIN-E AMONG BLOOD PLASMA LIPOPROTEINS IN HEALTHY MEN

DOI: https://doi.org/10.29296/24999490-2018-05-09

A.M. Kaneva, N.N. Potolitsyna, E.R. Bojko Institute of Physiology, Komi Science Center, Ural Branch of Russian Academy of Sciences, Pervomaiskaya av., 50, Syktyvkar, Komi Republic, 167982, Russian Federation E-mail: [email protected]

Introduction. Apolipoprotein-E (apoE), being one of the metabolically active apoproteins plays the important role in lipid metabolism. The aim of the study was to investigate the quantitative distribution of apoE between different lipoprotein classes in healthy men. Methods. The study was carried out in 36 practically healthy men aged 25–45 years. The plasma levels of apoE, total cholesterol, triglycerides and high-density lipoprotein cholesterol (HDL-C) were measured using the Powerwave 200 automated spectrophotometer (USA) with commercially available kits «Chronolab» (Switzerland). Along with the total plasma apoE level, the apoE concentration in high-density lipoproteins (apoE HDL) was determined. Amount of apoE in HDL fraction (% of apoE in HDL) was expressed as a percentage of the total plasma apoE level. Results. The quantitative distribution of apoE between different lipoprotein classes in the study subjects appeared to depend on the total plasma apoE level. At the low total plasma apoE level, the substantial amount of apoE was associated with HDL fraction. The increase of the total plasma apoE level was accompanied by the more even distribution of apoE between lipoprotein fractions. Meanwhile, the concentration of apoE in HDL fraction seemed to be stable and did not depend on the total plasma apoE level. Conclusion. The total plasma apoE level affects the quantitative distribution of apoE between different lipoprotein classes. At a decrease in the total plasma apoE level, the preservation of constant concentration of apoE in HDL due to its redistribution among lipoprotein classes appears to be prioritized.
Keywords: 
lipid metabolism, apolipoprotein-E, high density lipoproteins
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