SKIN FIBROBLASTS AS AN OBJECT FOR LIFE-TIME MOLECULAR DIAGNOSTIC OF THE ALZHEIMER DISEASE

DOI: https://doi.org/10.29296/24999490-2018-05-11

M.A. Paltsev(1), N.S. Linkova(2, 3), V.A. Zuev(2), V.O. Polyakova(2, 4, 5), N.N. Belushkina(1, 6), I.M. Kvetnoy(2, 4, 5) 1-Moscow State University M.V. Lomonosov, Faculty of Biology, Leninskie gory, 1/12, Moscow, 119991, Russian Federation; 2-Saint Petersburg Institute of Bioregulation and Gerontology, Dynamo pr., 3, Saint Petersburg, 197110, Russian Federation; 3-Peter the Great Saint Petersburg Polytechnic University, Polytechnicheskaya Str. 29, Saint Petersburg, 195251, Russian Federation; 4-D.O. Ott Research Institute of Obstetrics, Gynecology & Reproductology, Mendeleyevskaya line, 3, Saint Petersburg, 199034, Russian Federation; 5-St.Petersburg State University, Universitetskaya embankment, 7/9, Saint Petersburg, 199034, Russian Federation; 6-Institute of Biochemical physics named after N.M. Emanuel, Kosigina str., 4, Moscow, 119334, Russian Federation E-mail: [email protected]

Introduction. The Alzheimer disease (AD) is the socially important neurodegenerative disease. The promising way of the early lifetime diagnosis of the AD is the investigation of the expression of signal molecules by cells in a cerebrospinal fluid, blood lymphocytes, epitheliocytes and skin fibroblasts. The aim of the study was to establish the expression of signal molecules (Аβ42, τ-protein, protein kinase С, р53, р16) in skin fibroblasts managed to be relevant for the diagnosis in AD patients. Methods. Skin fibroblasts, obtained from elderly patients aged from 60 to 89 years. The control group included patients matched for age but without brain pathology. AD patients were diagnosed at the early and middle stage of presenile dementia accordingly to international criteria for the diagnosis of the AD: ICD-10-CM and NINCDS/ADRDA. The expression of signal molecules was studied with the use of immunofluorescent confocal microscopy method. Results. The expression of Аβ42, p53, p16 by skin fibroblasts obtained from old AD cases was shown to be higher than in patients of the same age without neurodegenerative pathology. Protein kinase C and τ-protein expression by skin fibroblasts obtained from elderly and old patients with or without AD didn’t differ.
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Conclusion. Skin fibroblasts seem to be the perspective object for the early lifetime diagnosis of the AD
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