STUDY OF EXPRESSION OF MMP-1 GENE IN LOCALIZED SСLERODERMIA

DOI: https://doi.org/10.29296/24999490-2019-02-04

Z. A. Nevozinskaya(5, 6), candidate of medical Sciences, A. G. Soboleva(1), candidate of biological Sciences, E. A. Klimov(3, 4), doctor of biological Sciences, I. M. Korsunskaya(1, 6), doctor of medical Sciences, Professor, V. V. Sobolev(1, 2, 3), candidate of biological Sciences 1-Center of theoretical problems of physico-chemical pharmacology of RAS, 4 Kosygina str., Moscow, 119991, Russian Federation; 2-RESEARCH Institute of vaccines and sera. I. I. Mechnikov", RAMS, 5 Malyy Kazenny per., Moscow, 105064, Russian Federation; 3-LLC "University diagnostic laboratory», Russian Federation, 119331, Moscow, Vernadsky Ave., 29, POM. I; 4-MOSCOW state University. M. V. Lomonosov, faculty of biology, Russian Federation, 119234, Moscow, Leninskie Gory, 1, p. 12; 5-Russian national research medical UNIVERSITY University them. N. And. Pirogov" Ministry Of Health Of Russia, 1 Ostrovityanova str., Moscow, 117997, Russian Federation; 6GBUZ "Moscow scientific and practical center of dermatovenerology and cosmetology" DZM, 17, Leninsky prospect, Moscow, 119071, Russian Federation E-mail: [email protected]

The aim of the study was to investigate the expression of the MMP-1 gene in patients with a diagnosis of localized scleroderma. Methods. The study included 12 patients with an established diagnosis of localized scleroderma. The material of the study consisted of biopsies from the affected and uninfected areas of the skin. As a control in the work, biopsies of the same patients from unaffected areas of the skin were used. The analysis was carried out by real-time PCR. The statistical processing of the results was carried out by the 2-ΔΔCt method. Results. The values of the relative expression levels of the gene were obtained in all patients. Individual analysis of each patient showed the expression level of the MMP-1 gene in scleroderma affected skin to be lowered comparatively with the visually unaffected skin (control) in the range from 4.2 times to 20.8 times. The average value of the change in the expression level of the gene was reduced by 7.6±2.6 times. Patients were divided into two groups – with a limited plaque form and a common plaque form of scleroderma. In patients with a limited form of the disease, expression of the MMP-1 gene was shown to be significantly reduced by 5.3 times, and in patients with a common form of the disease it was reduced by 11-fold times. Conclusion. A quantitative measurement of the expression of the metalloproteinase-1 gene (MMP-1) by PCR-RT was performed. Experimentally there was showed a decrease in gene expression in scleroderma affected skin compared to unaffected skin.
Keywords: 
scleroderma, gene expression, PCR-RT, MMP-1

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