THE SOME PECULIARITIES OF CAPSULAR FORMATION AFTER IMPLANTATION OF SILICONE WITH ADSORBED MULTIPOTENT MESENCHYMAL STROMAL CELLS

DOI: https://doi.org/10.29296/24999490-2019-02-08

I.V. Maiborodin(1), T.V. Mikheeva(1), S.A. Kuzkin(2), V.I. Maiborodina(2), A.I. Kadyrova(1), A.I. Shevela(1) 1-Center of New Medical Technologies, Institute of Chemical Biology and Fundamental Medicine, Akademika Lavrenteva pr., 8, Novosibirsk, 630090, Russian Federation; 2-Institute of Molecular Pathology and Pathomorphology of the Federal Research Center of Fundamental and Translational Medicine, Akademika Timakova str., 2, Novosibirsk, 630117, Russian Federation E-mail: [email protected]

Introduction. The widespread cellular technologies will sooner or later lead inevitably to the introduction of multipotent mesenchymal stromal cells (MMSC) or their exosomes to patients who have implanted artificial materials in their organism. The engraftment of foreign bodies is accompanied first by the acute and then chronic inflammation, very often acquiring a granulomatous character. A decrease in the activity of the inflammatory response can theoretically improve the results of implantation. Aim. To study the possibility of using autologous MMSC of bone marrow origin (AMMSCBMO) for the influence on the integration process of a silicone implant in the experiment. Methods. The condition of the capsule and tissues around the implanted silicone with adsorbed AMMSCBMO with a transfected GFP-gene and membranes stained by Vybrant® CM Dil was studied by methods of light microscopy with using luminescence. Results. When studying the thickness and vascularization of a capsule formed from dense fibrous connective tissue to delimiting the silicone implanted in the organism, no significant differences were found related to the adsorption of AMMSCBMO on the polymer. AMMSCBMO also do not affect the constriction processes of the capsule around the silicone and its fragmentation. The thickness of the loose fibrous connective tissue around the encapsulated silicone with adsorbed AMMSCBMO less; along with this the capsule contains more blood vessels, compared with the condition after the introduction of the same foreign body, but without the use of cellular technologies. Conclusion. A smaller volume of loose fibrous connective tissue around the implanted silicone with AMMSCBMO indicates a decrease in the activity of the inflammatory response as a result of the use of cellular technologies, more rapid cleansing of the postoperative wound from debris, the success of reparative processes and the creation of conditions for the integration of a foreign body into the organism. It is likely all found effects associated with the presence of AMMSCBMO on silicone after its implantation to be caused not by AMMSCBMO themselves, but by their detritus, which in some cases may have a similar immunomodulatory effect with AMMSCBMO themselves or even exceed it.
Keywords: 
multipotent mesenchymal stromal cells, silicone, inflammation, foreign body integration
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