COMPARATIVE ANALYSIS OF METHODS OF MITOCHONDRIAL REPLACEMENT THERAPY

DOI: https://doi.org/10.29296/24999490-2019-04-03

A.I. Glukhov(1, 2), A.M. Isagadzhiev(1) 1-I.M. Sechenov First Moscow State Medical University (Sechenov University), Trubetskaya st., 8, Moscow, 119991, Russian Federation; 2-M.V. Lomonosov Moscow State University, Leninskie Gory, 1–12, Moscow, 119234, Russian Federation E-mail: aiglukhov1958@gmail.com

Mitochondrial diseases are a group of inherited diseases characterized by the presence of defects in the mitochondrial mechanism of the patient. The distinguishing feature of such diseases is in transferring it via the maternal line to all the offspring. Various methods of mitochondrial replacement therapy are developed for the treatment of such diseases. Mitochondrial replacement therapy is a procedure which is used for the prevention of transferring such diseases via the maternal line to all descendants. There are several methods of this therapy. They are the following: pronuclear transfer, spindle transfer, polar body transfer 1 and 2. In this article, there are described modern used methods of mitochondrial replacement therapy. We should like to mention all the aspects of carrying out these procedures as well as complications and key features which must be taken into consideration when realizing them are also explained. We assessed the efficacy of these methods by comparing the data obtained by other researchers in the last 10 years. Having done such work we make a conclusion the most effective method of mitochondrial replacement therapy to be the polar body transfer 1. Besides it, we have revealed the spindle transfer to be also promising in spite of less prominent experimental results.
Keywords: 
mitochondria, heteroplasmy, mitochondrial diseases, mitochondrial replacement therapy

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