THE LEVEL OF EXPRESSION OF PROGESTERONE, ESTRADIOL AND VITAMIN D RECEPTORS IN MONONUCLEAR CELLS OF THE PERIPHERAL BLOOD OF PATIENTS WITH SURGICAL MENOPAUSE

DOI: https://doi.org/10.29296/24999490-2020-01-10

E.V. Kruchinina(1), O.S. Gorenkova(1), A.V Glazkova(1), E.Yu. Polyakova(2), N.A. Kochina(3), E.V. Krasnoshchok(3), Y.Z. Zaydieva(1), E.N. Kareva(3,4), S.Yu. Serebrova(3,5), 1-Moscow Regional Research Institute of Obstetrics and Gynecology, Pokrovka str, 22A, Moscow, 101000, Russian Federation; 2-M.F. Vladimirsky Moscow Regional Research Clinical Institute, Shchepkina str., 61/2, Moscow, 129110, Russian Federation; 3-N.I. Pirogov Russian National Research Medical University (RNRMU), st. Ostrovitianova 1, Moscow, 117997, Russian Federation; 4-I.M. Sechenov First Moscow State Medical University (Sechenov University), Trubetskaya str., 8/2, Moscow, 119991, Russian Federation; 5-Scientific Centre for Expert Evaluation of Medicinal Products, Petrovsky Blvd, 8/2, Moscow, 127051, Russian Federation E-mail: [email protected]

Introduction. Patients with surgical menopause are at risk for an osteopenic syndrome. For adequate medical correction of osteoporosis, it is necessary to clarify the mechanisms of the pathogenesis of this condition. It is known that osteoclasts, the activation of which is a recognized component of bone tissue destruction, are formed from circulating monocytes, and recently an active search is considered for ways to regulate this process. However, among monocytic marker molecules (the level of which varies in women with and without osteoporosis), there are no receptors or enzymes for steroid hormone metabolism. At the same time, the link between the level of sex steroids in women and the development of postmenopausal osteoporosis is obvious. The aim of the study. The level of transcription of possible target genes (estradiol and progesterone receptors, as well as vitamin D) was studied in peripheral blood mononuclear cells (PBMC). Methods. Densitometry, RT PCR. Results. It turned out that surgical menopause is characterized by manifestation of the osteopenic syndrome of the varying severity – from osteopenia to osteoporosis. At the same time, in patients with severe osteopenic syndrome (osteoporosis), there was a decrease in the expression of receptors for VDR, ER- alpha, and PR-A in PBMC by 11, 30 and 7 times, respectively (p≤0.05). Conclusion. Decrease in the expression of receptors for VDR, ER-alpha, and PR-A in PBMC can be regarded as one of the early pathogenesis of disorders of bone tissue metabolism caused by a deficiency of sex steroids.
Keywords: 
osteoporosis, mRNA, RT-PCR, peripheral blood mononuclear cell (PBMC)
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