STIMULATING EFFECT OF TETRAPEPTIDE AEDG ON MORPHOLOGIC AND FUNCTIONAL CHARACTERISTICS OF THE NERVOUS TISSUE

DOI: https://doi.org/10.29296/24999490-2020-04-07

N.I. Chalisova, N.G. Lopatina, N.G. Kamishev, T.G. Zachepilo Pavlov Institute of Physiology RAS, nab. Makarova 6, St. Petersburg, 199034, Russian Federation E-mail: [email protected]

Introduction. The investigation of biologic active substances, correcting the morphologic and functional characteristics of tissue changes, is an actual medicine task by the pathology therapy. Purpose of the study was the investigation of tetrapeptide AEDG effect on the nervous tissue of various organism species. Methods of organotypic culture of brain nervous tissue of Wistar line rats and conditioning of proboscis extension in honeybees Apis mellifera were used. Results. AEDG effect at 10-8 М concentration stimulates the cell proliferation in the rat brain nervous tissue, and at 10-5 М concentration stimulates the condition reflex preservation both in the honeybee short- and long term memory. Conclusion. The data about tetrapeptide AEDG stimulating effect on the cellular proliferation in the rat brain nervous tissue and on the function of the honeybee central nervous system can be used for creating preparations for treatment of this pathology of the nervous tissue. The parallelism of AEDG effect in two animal species resolves to suggest about the unique evolutionary mechanism of the peptide regulation in the living organism
Keywords: 
tetrapeptide, organotypic culture tissue, condition reflex, nervous tissue
Список литературы: 
  1. Havinson V.H., Kuznik B.I., Ryzhak G.A. Peptidnye bioreguljatory – novyj klass geroprotektorov. Uspehi gerontologii. 2013; 26 (1): 20–37.
  2. [Khavinson V.Kh , Kuznik B.I., Rigak G.A. Peptide bioregulators – new class of geroprotectors. Advances in Gerontology. 2013; 26 (1): 20–37 (In Russian)]
  3. Vanyushin B.F., Khavinson V.Kh. Short Biologically Active Peptides as Epigenetic Modulators of Gene Activity. Epigenetics – A Different Way of Looking at Genetics. W. Doerfler, P. Böhm (eds.). – Springer International Publishing Switzerland. 2016; 69–90.
  4. Chalisova N.I., Kontsevaja E.A., Vojtsehovskaja M.A., Ryzhak G.A. Vlijanie korotkih peptidov na razvitie organotipicheskoj kul'tury tkani kozhi molodyh i staryh krys. Profilakticheskaja i klinicheskaja meditsina. 2011; 2 (2): 110–3.
  5. [Chalisova N.I, Kontsevaya E.A., Voitzekhovskaya M.A., Rigak G. A.V. Effect of short peptides on the development of organotypic tissue culture of young and adult rat skin. Profilacticheskaya I klinicheskaya medicina. 2011; 2 (2): 110–3 (In Russian)]
  6. Chalisova N.I., Zhekalov A.N. Vlijanie dipeptida lejtsin-lizin na razvitie kul'tury tkani miokarda i selezenki. Uspehi gerontologii, 2014; 27 (3): 41–5. [Chalisova N.I, Zhekalov A. N.. Dipeptide Leucine-Lysin effect on the development of vuocard and spleen culture. Advances in Gerontology. 2014; 27 (3): 41–5 (In Russian)]
  7. Asok A., Leroy F., Rayman J.B., Kandel E.R. Molecular Mechanisms of the Memory Trace. Trends Neurosci. 2019; 42 (1): 14–22. https://doi.org/10.1016/j.tins.2018.10.005.
  8. Menzel R. The insect mushroom body, an experience-dependent recording device. J. Physiol Paris. 2014; 108 (2–3): 84–95. https://doi.org/10.1016/j.jphysparis.2014.07.004.
  9. Bitterman M.E., Menzel R., Fietz A., Schäfer S. Classical conditioning of proboscis extension in honeybees (Apis mellifera). J. Comp Psychol. 1983; 97 (2): 107–19.
  10. Lopatina N.G., Ryzhova I.V., Chesnokova E.G., Dmitrieva L.A. Retseptory L-glutamata v tsentral'noj nervnoj sisteme medonosnoj pchely Apis mellifera i ih rol' v protsesse formirovanija uslovnogo refleksa i sledov pamjati. Zhurnal evoljutsionnoj biohimii i fiziologii. 1997; 33 (6): 506–12.
  11. [Lopatina N.G., Rizhova I.V., Chesnokova E.G., Dmitrieva L.A. L-glutamate receptors in central nervous system of honey-bee Apis mellifera and its role at the process of the conditional reflex and memory tracks development. 1997; 33 (6): 506–12 (In Russian)]
  12. Havinson V.H., Lopatina N.G., Chalisova N.I., Zachepilo T.G., Lin'kova N.S., Halimov R.I., Kamyshev N.G. Tripeptid moduliruet uslovno-reflektornuju dejatel'nost' medonosnoj pchely Apis mellifera L. Fundamental'nye issledovanija. 2015; 2: 491–6.
  13. [Khavinson V.Kh., Lopatina N.G., Chalisova N.I., Zachepilo T.G., Linkova N.S., Khalimov R.I, Kamishev N.G. L Tripeptide modulates the conditional reflex action in honey-bee Apis mellifera. Fundamental Researches. 2015; 2: 491–6 (In Russian)]
  14. Khavinson V., Micans P., Maryanovich A. Peptides in the Epigenetic Control of Ageing. Great Britain: Profound Health Ltd. 2017; 116.