SINGLE-PHOTON EMISSION COMPUTERIZED TOMOGRAPHY WITH 99mTc-DARPin9_29 IN DIAGNOSTICS OF BREAST CANCER WITH Her2/neu OVEREXPRESSION: FIRST CLINICAL EXPЕRIENCE

DOI: https://doi.org/10.29296/24999490-2021-01-06

1-Cancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences, Kooperativny Street, 5, Tomsk, 634009, Russian Federation; 2-National Research Tomsk Polytechnic University, Lenina Avenue, 30, Tomsk, 634050, Russian Federation; 3-Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Ul. Miklukho-Maklaya, 16/10, Moscow, 117997, Russian Federation; 4-Uppsala University, Sweden, Uppsala, Segerstedthuset, Dag Hammarskjölds väg 7 E-mail: [email protected]

Introduction. In recent years, alternative framework proteins are used for the targeted radionuclide imaging. Molecules of DARPin (Design Ankyrin Repeat Protein) are one of the representatives of scaffolds. Despite the continued increase in the number of molecular targets in oncological practice, the epidermal growth receptor Her2/neu is still of great interest, the overexpression of which is most often observed in patients with breast cancer and accounts for 15–20% of cases. However, the methods of the determination of Her2/neu have many significant drawbacks. The aim of the study. Assessment of the 99mTc-DARPin9_29 diagnostic efficacy for the diagnosis of breast cancer (BC) with Her2/neu overexpression. Methods. The study included 8 BC cancer patients (T1-4N0-2M0) who were not receiving systemic therapy at the time of the study: in 4 patients, Her2/neu overexpression was noted, in 4 patients – not detected. At the preclinical stage, all patients underwent morphological and immunohistochemical studies of the primary tumor biopsy material. Radiopharmaceutical 99mTc-DARPin9_29 was injected intravenously, WholeBody scintigraphy and SPECT were performed 2 hours after injection. Results. The distribution of radiopharmaceuticals in organs 2 hours after injection revealed the greatest accumulation in the liver and kidneys (26.28 and 6.68% of the administered dose, respectively). In studying tumor/background indices there were revealed values of the studied parameter in patients with overexpression of Her2 receptors to be more than by 2.8 times higher than the values in the subgroup of patients with negative expression of this marker. Conclusion. According to the results obtained at the initial stages of the study, it can be said that the 99mTc-DARPin9_29 preparation can be considered as a promising additional method for the diagnosis of BC with overexpression of the Her2 / neu receptor in the future.
Keywords: 
breast cancer, DARPin9_29, target radionuclide diagnostics
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