PROTECTIVE EFFECT OF CODED L-AMINO ACIDS IN CYTOSTATIC PRESENCE ON THE DEVELOPMENT OF ORGANOTYPIC CULTURE OF TISSUES OF DIFFERENT GENESIS

DOI: https://doi.org/10.29296/24999490-2021-02-07

N.I. Chalisova(1, 2), G.A. Ryzhak(1), O.M. Ivko(1), E.S. Zalomaeva(1–3), P.N. Ivanova(1, 2) 1-St. Petersburg Institute of Bioregulation and Gerontology, pr. Dinamo, 3, St. Petersburg, 197110, Russian Federation; 2-Pavlov Institute of Physiology RAS, nab. Makarova, 6, St. Petersburg, 199034, Russian Federation; 3-Russian State Pedagogical University. A.I. Herzen, St. Petersburg, 191186, nab. Moiki, 48 E-mail: ni_chalisova@mail.ru

Introduction. The cytostatics, inhibiting the cellular proliferation, are using in clinic for the inhibition of tumor development in patients. However, the adverse events can occur because of the damage of health cells of other tissues. A delay or decrease of these effects is an actual medicine task. Purpose of the study was the investigation of effects of coded L-amino acids on a decrease of cytostatic inhibiting effects in the culture of tissues of different genesis. These amino acids are not only the structural elements of the proteins, but can participate in the regulation of the genes, controlling the cellular cycle. Methods of organotypic culture of tissues of brain cortex, muscle, bladder of Wistar line rats in presence of cytostatic cyclophosphane (CP) and also of L-amino acids were used. The combined effect of CP and L-amino acids, stimulating the cellular regeneration, the cytostatic inhibiting effect on the cellular proliferation in the tissues of different genesis decreased by 10–43%. Conclusion. The data obtained about protective effect of coded hydrophile L-amino acids by cytostatic effect creates the base for development of medications for the delay of adverse effects related to the chemotherapy.
Keywords: 
L-amino acids, cytostatic, organotypic culture tissue, cellular proliferation

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