Features of local expression of mRNA, IL-1β, IL-18, CCL2/MCP-1 in modeling pigment epithelium atrophy and retinal degeneration in the experiment on rabbits

DOI: https://doi.org/10.29296/24999490-2021-02-08

V.V. Neroev, N.V. Balatskaya, E.V. Svetlova, N.V. Neroeva, M.V. Ryabina, A.G. Karmokova, O.A. Losanova, I.Yu. Chernomorets, P.A. Ilyukhin Helmholtz National Medical Research Center of Eye Diseases, Ministry of Health of Russia, Sadovaya-Chernogryazskaya, 14/19, Moscow, 105062, Russian Federation Е-mail: asyakarma17@gmail.com

Introduction. Degenerative retinal diseases are considered as the leading cause of blindness and low vision worldwide. The last studies have provided convincing evidence of the involvement of immunological factors in the development and progression of the disease. Meanwhile the immunopathogenesis of the wet form of AMD has been studied more fully than the atrophic one. The aim of the study. Study of local expression of mRNA of inflammatory cytokines IL-1β, IL-18, CCL2/MCP-1 in a model of RPE atrophy. Material and methods. The material for the study was samples of the retinal-RPE-choroid (TC) tissue complex isolated at various times from the enucleated rabbits’ eyes with previously created RPE atrophy by subretinal injection of 0,9% sodium chloride solution. Optical coherence tomography (OCT) and autofluorescence (AF) study using HeidelbergSpectralis ™ SD-OCT were performed. Local expression of cytokines was determined by RT-PCR. Results. A single subretinal injection of 0.01 ml of 0.9% sodium chloride solution was found to be associated with multidirectional changes in the expression of mRNA of the IL-1β, IL-18, MCP-1/CCL2 genes relative to the norm. Three types of the retinal response, formed during the development of atrophic changes and determined by the degree of local mRNA expression of cytokine genes, were characterized. No correlation was found between the magnitude of local mRNA expression to the stimulus inducing atrophy and the size of the atrophic retinal lesion. Conclusion. Obtained data can be helpful in the research of RPE atrophy and in the stem cells treatment.
Keywords: 
atrophy of retinal pigment epithelium, retinal degeneration, tissue complex retina – choroid, inflammatory mediators, gene expression, RT-PCR in real time

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