EFFECT OF TRIPEPTIDES ON GROWTH OF ORGANOTYPIC CULTURE OF DIFFERENT GENESIS TISSUES

DOI: https://doi.org/https://doi.org/10.29296/24999490-2022-03-04

N.I. Chalisova(1, 2), G.A. Ryzhak(2), R.S. Umnov(2), N.S. Linkova(2, 3)
1-Pavlov Institute of Physiology RAS, Makarova emb., 6, Saint Petersburg, 199034, Russian Federation;
2-Saint Petersburg Institute of Bioregulation and Gerontology, Dynamo pr., 3, Saint Petersburg, 197110, Russian Federation;
3-Belgorod National Research University, Pobedy str., 85, Belgorod, 308009, Russia

Tripeptides are physiological products of protein degradation and informational molecules. AED (Ala-Glu-Asp), EDR (Glu-Asp-Arg), EDG (Glu-Asp-Gly) peptides have geroprotective, neuroprotective, reparative and antioxidant properties. The aim of the work is to evaluate the effect of the AED, EDR, EDG peptides on the development of organotypic tissue culture of various genesis in young and old rats. Material and methods. Tissues for organotypic cultivation of mesodermal genesis (testis), ectodermal genesis (cerebral cortex) and entodermal genesis (bladder) were obtained from young (2–3 months) and old (16–18 months) Wistar rats. Results. AED peptide stimulated cell growth in meso- and endodermal tissues of young and old animals. EDR peptide activated cell proliferation in ecto- and mesodermal tissues of young and old rats. EDG peptide had a stimulating effect on the mesodermal tissue of young rats. Conclusion. Tripeptides, depending on the composition and sequence of their amino acids, have different effects on meso-, ecto- and endodermal genesis tissues obtained from animals of different ages. This indicates the possibility of targeted geroprotective action of AED, EDR, EDG peptides.
Keywords: 
tripeptides, organotypic cell tissue, tissues of various genesis, aging
Список литературы: 
  1. Хавинсон В.Х. Лекарственные пептидные препараты: прошлое, настоящее, будущее. Клиническая медицина. 2020; 98 (3): 165–77. https://doi.org/10.30629/0023-2149-2020-98-3-165-177. https://doi.org/10.30629/0023-2149-2020-98-3-165-177 [Khavinson V.Kh. Medicinal peptide drugs: past, present, future. Clinical Medicine. 2020; 98 (3): 165–77. https://doi.org/10.30629/0023-2149-2020-98-3-165-177. (In Russian)].
  2. Хавинсон В.Х., Попович И.Г., Рыжак Г.А., Сафарова Г.Л., Вершинина Е.А. Лекарственные пептидные препараты в аспекте доказательной медицины. Патогенез. 2021; 19 (1): 19–29. https://doi.org/10.25557/2310-0435.2021.01.19-29. [Khavinson V.Kh., Popvich I.G., Ryzhak G.A., Safarova G.L., Vershinina E.A. Medicinal peptide preparations in the aspect of evidence-based medicine. Pathogenesis. 2021; 19 (1): 19–29. https://doi.org/10.25557/2310-0435.2021.01.19-29. (In Russian)].
  3. Журкович И.К., Ковров Н.Г., Рыжак Г.А., Миронова Е.С., Хавинсон В.Х. Идентификация коротких пептидов в составе полипептидных комплексов, выделенных из органов животных. Успехи современной биологии. 2020; 140 (2): 140–8. https://doi.org/10.31857/S004213242002012X. [Zhurkovich I.K., Kovrov N.G., Ryzhak G.A., Mironova E.S., Khavinson V.Kh. Identification of short peptides in the composition of polypeptide complexes isolated from animal organs. Biology Bulletin Review. 2020; 140 (2): 140–8. https://doi.org/10.31857/S004213242002012X. (In Russian)].
  4. 4. Khavinson V.Kh., Popovich I.G., Linkova N.S., Mironova E.S., Ilina A.R. Peptide Regulation of Gene Expression: A Systematic Review. Molecules. 2021; 26: 1–22. https://doi.org/10.3390/molecules26227053.
  5. Ashapkin V., Khavinson V., Shilovsky G., Linkova N., Vanuyshin B. Gene expression in human mesenchymal stem cell aging cultures: modulation by short peptides. Molecular Biology Reports. 2020; 47: 4323–9. https://doi.org/10.1007/s11033-020-05506-3.
  6. Chalisova N.I. Effect of synthesized dipeptide L-Leu-Lys on cell proliferation and apoptosis in organotypic tissue culture from rat spleen. Applied Cell Biology. 2015; 4 (2): 27–31.
  7. Khavinson V.Kh., Linkova N.S., Dudkov A.V., Polyakova V.O., Kvetnoi I.M. Peptidergic Regulation of Expression of Genes Encoding Antioxidant and Anti-Inflammatory Proteins. Bull. Exp. Biol. Med. 2012; 152 (5): 615–8. https://doi.org/10.1007/s10517-012-1590-2.
  8. Фридман Н.В., Линькова Н.С., Бойко Л.В., Кахели М.А. Влияние пептидных биорегуляторов на структурно-функциональные особенности кожи лица женщин пожилого возраста. Молекулярная медицина. 2021; 4: 42–6. https://doi.org/10.29296/24999490-2021-04-07. [Fridman N.V., Linkova N.S., Bijko L.V., Kacheli M.A. The effect of peptide bioregulators on the structural and functional features of the facial skin of elderly women. Molecular Medicine. 2021; 4: 42–6. https://doi.org/10.29296/24999490-2021-04-07 (In Russian)].
  9. Khavinson V., Ilina A., Kraskovskaya N., Linkova N., Kolchina N., Mironova E., Erofeev A., Petukhov M. Neuroprotective Effects of Tripeptides—Epigenetic Regulators in Mouse Model of Alzheimer’s Disease. Pharmaceuticals. Special Issue Epigenetic Drugs. 2021; 14 (6): 515. https://doi.org/10.3390/ph14060515.
  10. Khavinson V., Ribakova Y., Kulebiakin K., Vladychenskaya E., Kozina L., Arutjunyan A., Boldyrev A. Pinealon increases cell viability by suppression of free radical levels and activating proliferative processes. Rejuvenation Res. 2011; 14 (5): 535–41. https://doi.org/10.1089/rej.2011.1172.
  11. Fridman N.V., Linkova N.S., Kozhevnikova E.O., Gutop E.O. Khavinson V.Kh. Comparison of the effects of KE and AED peptides on functional activity of human skin fibroblasts during their replicative aging. Bull. Exp. Biol. Med. 2020; 3: 154–7. https://doi.org/0.47056/1814-3490-2020-3-197-201.
  12. Рыжак Г.А., Чалисова Н.И., Ивко О.М., Заломаева Е.С., Иванова П.Н. Протекторное влияние биорегуляторных пептидов на развитие органотипической культуры тканей в присутствии цитостатика. Молекулярная медицина. 2020; 18 (6): 52–6. https://doi.org/10.29296/24999490-2020-06-08. [Ryzhak G.A., CHalisova N.I., Ivko O.M., Zalomaeva E.S., Ivanova P.N. Protective effect of bioregulatory peptides on the development of organotypic tissue culture in the presence of cytostatics. Molekulyarnaya medicina. 2020; 18 (6): 52–6. https://doi.org/10.29296/24999490-2020-06-08. (In Russian)].