EXPERIMENTAL STUDY OF CRYSTAL STRUCTURES OBSERVED ON THE SURFACEOF THE DENTINE WITH THE USE SCANNING ELECTRON MICROSCOPY

N.O. Bessudnova, D.I. Bilenko, S.B. Venig, V.S. Atkin, V.V. Galuska, A.M. Zakharevich
Federal State Budgetary Educational Institution of Higher Professional Education National Research University Saratov State University named after N.G. Chernyshevsky

In a series of laboratory experiments on the surface of dentin treated with etching gel – 38% Phosphoric Acid, and exposed to 95% ethanol solution for 30 days at ambient temperature the crystal structures have been found. Three-Dimensional Visualization of these superficial masses has been performed with the use of electronic scanning microscopy and aid Alicona 3D MeX software (Alicona GmbH). Metric characteristics of the crystals have been calculated. Methods of X-ray spectral microanalysis were used to determine the chemical elements composition of the dentinal substrate and crystals, the latters turned out to be calcium-phosphate compounds. The mechanism of the formation of Calcium phosphate crystals on the dentin surface defects due to the diffusion of substances in solution has been described. The formation and growth of crystals were found to take place by the way of the eventual redistribution of ions in dentin material.
Keywords: 
dentin, formation and growth of crystals, visualization, calcium phosphate compounds, brushite, scanning electron microscopy, X-ray spectral analysis