MOLECULAR CHARACTERIZATION OF UTERINE LEIOMYOMA ACCORDINGTO EXPRESSION OF GENES OF STEROID RECEPTORS, STS, SULT AND PTEN

S.V. Shramko (1), A.S. Leshchenko (2), E.P. Khvostova (3), M.A. Sabantsev (1), L.G. Bazhenova (4), L.F. Gulyaeva (2,3)
1 -City Clinical Hospital № 1, Novokuznetsk
2 -Novosibirsk State University
3 -Institute of Molecular Biology and Biophysics, Novosibirsk
4 -Novokuznetsk Postgraduate Physician Institute

Leiomyoma is one of the most common benign tumors of the female reproductive system. As this pathology in most cases is hormone dependent, we determined the level of gene expression of steroid receptors ERs, PR, estrogen sulfotransferase SULT1E1, STS steroid sulphatase and tyrosine phosphatase PTEN in myoma nodes in 33 women using Real-time RT-PCR. A significant increase in the level of mRNA of the ERα, ERβ, PgR, and the PTEN gene in samples from proliferative and non-proliferative uterine leiomyoma, as well as the decrease in the expression of genes STS, PTEN and PgR in samples of myoma associated with hyperplasia and malignant uterine have been identified.
Keywords: 
uterine cancer, estrogen receptor, progesterone receptor, steroid sulfatase, steroid sulfotransferase, PTEN